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1'-hydroxy-2'-oxopropyltetrahydropterin + NADP+
L-erythro-tetrahydrobiopterin + NADPH
-
-
-
?
1'-hydroxy-2'-oxopropyltetrahydropterin + NADPH
L-erythrotetrahydrobiopterin + NADP+
-
-
-
?
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin + NADP+
D-sepiapterin + NADPH
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin + NADPH
tetrahydrodictyopterin + NADP+
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+
L-sepiapterin + NADPH
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADPH
L-erythro-tetrahydrobiopterin + NADP+
1,2-cyclohexadione + NADPH
?
-
-
-
?
1,2-naphthoquinone + NADPH
?
-
-
-
?
1,4-naphthoquinone + NADPH
?
-
-
-
?
1-phenyl-1,2-propanedione + NADPH
?
-
-
-
?
2,3-butanediol + NADP+
?
-
-
-
?
2,3-dimethoxynaphthalene-1,4-dione + NADPH
?
-
-
-
?
2,4-pentanedione + NADPH
?
-
-
-
?
2-amino-6-(2-hydroxypropanoyl)pteridin-4(1H)-one + NADPH + H+
?
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
6-pyruvoyl tetrahydropterin + NADPH
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+
6-pyruvoyl tetrahydropterin + NADPH
L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+
6-pyruvoyl tetrahydropterin + NADPH + H+
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+
6-pyruvoyl tetrahydropterin + NADPH + H+
L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
6-pyruvoyl tetrahydropterin + NADPH + H+
tetrahydrobiopterin + NADP+
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH
tetrahydrobiopterin + NADP+
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+
L-erythro-tetrahydrobiopterin + 2 NADP+
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+
L-threo-tetrahydrobiopterin + 2 NADP+
6-pyruvoyltetrahydropterin + NADPH
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+
-
-
no synthesis of the D-isomer
?
9,10-phenanthrenequinone + NADP+
?
-
-
-
?
9,10-phenanthrenequinone + NADPH
?
-
-
-
?
acetohexamide + NADPH
?
-
-
-
?
acetophenone + NADPH
?
-
-
-
?
benzylacetone + NADPH
?
-
-
-
?
diacetyl + NADPH
acetoin + NADP+
-
NADPH-dependent dicarbonyl reductase activity
-
?
dihydrotepidopterin + NADP+
?
-
15% relative activity
-
?
isosepiapterin + NADPH
6-(1-hydroxypropyl)-dihydropterin + NADP+
-
-
-
?
isosepiapterin + NADPH
? + NADP+
-
-
-
r
L-erythro-dihydrobiopterin + NADP+
?
-
-
-
?
L-erythro-dihydroneopterin + NADP+
?
L-threo-dihydrobiopterin + NADP+
?
-
D-isomer 45% relative activity
-
r
L-threo-dihydroneopterin + NADP+
?
lactoyltetrahydropterin + NADPH
L-erythrotetrahydrobiopterin + NADP+
-
-
-
?
lactoyltetrahydropterin + NADPH + H+
L-erythro-tetrahydrobiopterin + NADP+
-
-
-
?
p-nitrobenzaldehyde + NADPH
p-nitrobenzyl alcohol + NADP+
p-quinone + NADPH
?
-
-
-
?
phenylpropanedione + NADPH
phenylpropan-2,3-diol + NADP+
-
NADPH-dependent dicarbonyl reductase activity
-
?
propiophenone + NADPH
?
-
-
-
?
pyruvoyltetrahydropterin + NADPH
L-erythrotetrahydrobiopterin + NADP+
-
-
-
?
sepiapterin + NADPH
1'-hydroxy-2'-oxopropyltetrahydropterin + NADP+
-
best substrate
-
?
sepiapterin + NADPH
7,8-dihydrobiopterin + NADP+
-
-
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
sepiapterin + NADPH + H+
tetrahydrobiopterin + NADP+
xanthopterin B2 + NADPH
6-(1',2'-dihydroxypropyl)-dihydrolumazine + NADP+
-
-
-
?
xanthopterin-B2 + NADPH
? + NADP+
-
-
-
r
additional information
?
-
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin + NADP+

D-sepiapterin + NADPH
-
-
i.e. 1'-oxo-2'-D-hydroxypropyl-dihydropterin
?
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin + NADP+
D-sepiapterin + NADPH
-
-
i.e. 1'-oxo-2'-D-hydroxypropyl-dihydropterin
?
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin + NADPH

tetrahydrodictyopterin + NADP+
-
-
-
?
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin + NADPH
tetrahydrodictyopterin + NADP+
-
preferred substrate
-
?
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin + NADPH
tetrahydrodictyopterin + NADP+
-
-
-
?
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin + NADPH
tetrahydrodictyopterin + NADP+
-
preferred substrate
-
?
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+

L-sepiapterin + NADPH
-
-
i.e. 1'-oxo-2'-L-hydroxypropyl-dihydropterin
?
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+
L-sepiapterin + NADPH
-
-
i.e. 1'-oxo-2'-L-hydroxypropyl-dihydropterin
?
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADPH

L-erythro-tetrahydrobiopterin + NADP+
-
-
-
?
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADPH
L-erythro-tetrahydrobiopterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH

6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
in the reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding L-isomers are inactive
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
equilibrium lies much in favor of dihydrobiopterin formation
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
in the reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding L-isomers are inactive
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
equilibrium lies much in favor of dihydrobiopterin formation
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
equilibrium lies much in favor of dihydrobiopterin formation
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
catalyzes the complete reduction of pyrovoyltetrahydropterin to tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-pyruvoyl tetrahydropterin + NADPH

1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+
-
-
-
?
6-pyruvoyl tetrahydropterin + NADPH
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+
-
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin is formed by an aldose reductase-like enzyme activity, not by SR
-
?
6-pyruvoyl tetrahydropterin + NADPH

L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+
-
last step of the de novo tetrahydrobiopterin biosynthesis initiating from GTP
-
?
6-pyruvoyl tetrahydropterin + NADPH
L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+
-
last step of the tetrahydrobiopterin biosynthesis
-
?
6-pyruvoyl tetrahydropterin + NADPH
L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+
-
substrate binding mode for stereospecific production of L-threo-tetrahydrobiopterin, structure-function relationship
-
?
6-pyruvoyl tetrahydropterin + NADPH
L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+
-
two reductive steps oxidizing NADPH
-
?
6-pyruvoyl tetrahydropterin + NADPH + H+

1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+
-
-
-
?
6-pyruvoyl tetrahydropterin + NADPH + H+
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+
-
-
-
?
6-pyruvoyl tetrahydropterin + NADPH + H+

tetrahydrobiopterin + NADP+
-
-
-
?
6-pyruvoyl tetrahydropterin + NADPH + H+
tetrahydrobiopterin + NADP+
-
final step in the synthesis of tetrahydrobiopterin, role in detoxification of exogenous carbonyl compounds suggested
-
?
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH

tetrahydrobiopterin + NADP+
-
-
-
r
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH
tetrahydrobiopterin + NADP+
-
-
-
r
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH
tetrahydrobiopterin + NADP+
-
-
the cofactor for aromatic amino acid hydrolases in the biosynthesis of aromatc amino acids and dopamine
r
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH
tetrahydrobiopterin + NADP+
-
-
-
r
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+

L-erythro-tetrahydrobiopterin + 2 NADP+
-
-
-
?
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+
L-erythro-tetrahydrobiopterin + 2 NADP+
-
-
-
?
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+
L-erythro-tetrahydrobiopterin + 2 NADP+
-
-
-
?
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+

L-threo-tetrahydrobiopterin + 2 NADP+
-
-
no formation of the L-erythro isomer
?
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+
L-threo-tetrahydrobiopterin + 2 NADP+
-
final step in the synthesis of tetrahydrobiopterin
no formation of the L-erythro isomer
?
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+
L-threo-tetrahydrobiopterin + 2 NADP+
-
no formation of the L-erythro isomer
?
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+
L-threo-tetrahydrobiopterin + 2 NADP+
final step in the synthesis of tetrahydrobiopterin
no formation of the L-erythro isomer
?
acetoin + NADPH

?
-
-
-
?
acetoin + NADPH
?
-
0.003 mM NADPH oxidised per 30 min
-
?
benzil + NADPH

?
-
-
-
?
benzil + NADPH
?
-
NADPH-dependent dicarbonyl reductase activity
-
?
diacetyl + NADPH

?
-
-
-
?
diacetyl + NADPH
?
-
-
-
?
diacetyl + NADPH
?
-
-
-
?
diacetyl + NADPH
?
-
-
-
?
L-erythro-dihydroneopterin + NADP+

?
-
D-isomer completely inactive
-
r
L-erythro-dihydroneopterin + NADP+
?
-
very slow activity, no activity with the D-isomer
-
r
L-threo-dihydroneopterin + NADP+

?
-
-
-
?
L-threo-dihydroneopterin + NADP+
?
-
D-isomer completely inactive
-
r
L-threo-dihydroneopterin + NADP+
?
-
very slow activity, no activity with the D-isomer
-
r
menadione + NADPH

?
-
-
-
?
menadione + NADPH
?
-
-
-
?
menadione + NADPH
?
-
-
-
?
menadione + NADPH
?
-
-
-
?
methylglyoxal + NADPH

?
-
-
-
?
methylglyoxal + NADPH
?
-
-
-
?
methylglyoxal + NADPH
?
-
-
-
?
p-nitrobenzaldehyde + NADPH

p-nitrobenzyl alcohol + NADP+
-
-
-
?
p-nitrobenzaldehyde + NADPH
p-nitrobenzyl alcohol + NADP+
-
-
-
?
phenylglyoxal + NADPH

?
-
-
-
?
phenylglyoxal + NADPH
?
-
-
-
?
phenylglyoxal + NADPH
?
-
-
-
?
phenylglyoxal + NADPH
?
-
-
-
?
sepiapterin + NADPH + H+

7,8-dihydrobiopterin + NADP+
-
-
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
r
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
r
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
r
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
cellular uptake of sepiapterin across the cell membrane results in an efficient accumulation of tetrahydrobiopterin, sepiapterin relocates across the plasma membrane via two distinct paths, the slower path connects to the cytosolic compartment, overview
-
r
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
no stereochemic specification in the publication
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
r
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
r
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
detailed product determination and analysis using fluorescent substrates, 7,8-dihydrobiopterin can be oxidized nonenzymatically to biopterin
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
i.e. 2-amino-4-hydroxy-6-lactyl-7,8-dihydropteridine, biosynthesis pathway, overview
i.e. 2-amino-4-hydroxy-6-(1',2'-dihydroxypropyl-)7,8-dihydropteridine, which is a substrate for biosynthesis of tetrahydrobiopterin, i.e. 6R-L-erythro-1',2'-dihydroxy-propyl-2-amino-4-hydroxy-5,6,7,8-tetrahydropteridine, an important component for controling the concentration of catecholamines or indoleamines in the brain and other nerve tissues
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
sepiapterin reductase activity
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
no stereochemic specification in the publication
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
?
sepiapterin + NADPH + H+

tetrahydrobiopterin + NADP+
-
no stereochemic specification in the publication
?
sepiapterin + NADPH + H+
tetrahydrobiopterin + NADP+
-
-
no stereochemic specification in the publication
?
sepiapterin + NADPH + H+
tetrahydrobiopterin + NADP+
-
no stereochemic specification of the substrate in the publication
?
sepiapterin + NADPH + H+
tetrahydrobiopterin + NADP+
-
no stereochemic specification in the publication
?
additional information

?
-
-
not: methylglyoxal, menadione
-
?
additional information
?
-
-
product determination
-
?
additional information
?
-
-
D-threo-tetrahydrobiopterin is synthesized via 1'-oxo-2'-D-hydroxypropyl-tetrahydropterin in Dictyostelium discoideum strain Ax2
-
?
additional information
?
-
-
quantitative in vivo assay and product determination
-
?
additional information
?
-
-
tetrahydropteridines in Dictyostelium are associated with mitochondrial function, probably via direct protection against oxidative stress, tetrahydropteridine deficiency impairs mitochondrial function in Dictyostelium discoideum Ax2
-
?
additional information
?
-
-
the enzyme catalyzes the final step in the tetrahydrobiopterin biosynthesis
-
?
additional information
?
-
-
product determination
-
?
additional information
?
-
-
quantitative in vivo assay and product determination
-
?
additional information
?
-
-
D-threo-tetrahydrobiopterin is synthesized via 1'-oxo-2'-D-hydroxypropyl-tetrahydropterin in Dictyostelium discoideum strain Ax2
-
?
additional information
?
-
-
not: 6-acetyl-7-methyldihydropterin
-
?
additional information
?
-
-
no activity with 6-acetyl-7-methyl-7,8-dihydropterin
-
?
additional information
?
-
-
final step in the synthesis of tetrahydrobiopterin
-
?
additional information
?
-
-
the enzyme is involved in the tetrahydrobiopterin biosynthesis
-
?
additional information
?
-
-
sepiapterin or dihydrobiopterin are the precursors of the tetrahydrobiopterin-salvage pathway, enzyme activity and pathway regulation in vivo, overview
-
?
additional information
?
-
-
the enzyme catalyzes the final step in the tetrahydrobiopterin biosynthesis
-
?
additional information
?
-
-
the enzyme catalyzes the final step in the tetrahydrobiopterin biosynthesis, sepiapterin reductase expression is increased in Parkinsons disease brain tissue
-
?
additional information
?
-
native ornithine decarboxylase, EC 4.1.1.17, and sepiapterin reductase physically interact, in silico protein-protein docking simulations
-
?
additional information
?
-
-
native ornithine decarboxylase, EC 4.1.1.17, and sepiapterin reductase physically interact, in silico protein-protein docking simulations
-
?
additional information
?
-
physical interaction between key polyamines biosynthesis enzyme ornithine decarboxylase (ODC) and sepiapterin reductase (SPR)
-
?
additional information
?
-
-
physical interaction between key polyamines biosynthesis enzyme ornithine decarboxylase (ODC) and sepiapterin reductase (SPR)
-
?
additional information
?
-
the enzyme's one-electron reduction of redox cycling chemicals, such as menadione, generates radical cations. The rapid reaction of these cations with molecular oxygen regenerates the redox active chemical, and in the process superoxide anion is formed. Sepiapterin reduction is catalyzed by NADPH, whereas chemical redox cycling is catalyzed by NADPH and/or NADH, overview
-
?
additional information
?
-
-
the enzyme's one-electron reduction of redox cycling chemicals, such as menadione, generates radical cations. The rapid reaction of these cations with molecular oxygen regenerates the redox active chemical, and in the process superoxide anion is formed. Sepiapterin reduction is catalyzed by NADPH, whereas chemical redox cycling is catalyzed by NADPH and/or NADH, overview
-
?
additional information
?
-
-
final step in the synthesis of tetrahydrobiopterin
-
?
additional information
?
-
-
important in the metabolism of tetrahydrobiopterin and sepiapterin
-
?
additional information
?
-
-
enzyme is quite similar to general aldo-keto reductases, especially to carbonyl reductase
-
?
additional information
?
-
-
some monoketotestosterones show slight activity
-
?
additional information
?
-
-
not: 6-acetyl-7-methyldihydropterin
-
?
additional information
?
-
-
enzyme also catalyzes the isomerization of 6-1'-oxo-2'-hydroxypropyl tetrahydropterin (6-lactoyltetrahydropterin) to 6-1'-hydroxy-2'-oxopropyltetrahydropterin
-
?
additional information
?
-
-
final step in the synthesis of tetrahydrobiopterin
-
?
additional information
?
-
-
the enzyme might be involved in regulation of biosynthesis of catecholamines, indoleamines, and tetrahydrobiopterins
-
?
additional information
?
-
-
descending substrate preference: sepiapterin, lactoyltetrahydropterin, pyruvoyltetrahydropterin, 1'-hydroxy-2'-oxopropyltetrahydropterin
-
?
additional information
?
-
-
substrate specificity, the enzyme also exhibits non-specific NADPH-dependent dicarbonyl reductase activity, overview
-
?
additional information
?
-
the enzyme's one-electron reduction of redox cycling chemicals, such as menadione, generates radical cations. The rapid reaction of these cations with molecular oxygen regenerates the redox active chemical, and in the process superoxide anion is formed. Sepiapterin reduction is catalyzed by NADPH, whereas chemical redox cycling is catalyzed by NADPH and/or NADH, overview
-
?
additional information
?
-
-
the enzyme might be involved in regulation of biosynthesis of catecholamines, indoleamines, and tetrahydrobiopterins
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin + NADP+
D-sepiapterin + NADPH
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin + NADPH
tetrahydrodictyopterin + NADP+
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+
L-sepiapterin + NADPH
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADPH
L-erythro-tetrahydrobiopterin + NADP+
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
6-pyruvoyl tetrahydropterin + NADPH
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+
-
-
-
-
?
6-pyruvoyl tetrahydropterin + NADPH
L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+
6-pyruvoyl tetrahydropterin + NADPH + H+
tetrahydrobiopterin + NADP+
-
final step in the synthesis of tetrahydrobiopterin, role in detoxification of exogenous carbonyl compounds suggested
-
-
?
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH
tetrahydrobiopterin + NADP+
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+
L-threo-tetrahydrobiopterin + 2 NADP+
6-pyruvoyltetrahydropterin + NADPH
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+
-
-
no synthesis of the D-isomer
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
sepiapterin + NADPH + H+
tetrahydrobiopterin + NADP+
additional information
?
-
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin + NADP+

D-sepiapterin + NADPH
-
-
-
-
?
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin + NADP+
D-sepiapterin + NADPH
-
-
-
-
?
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin + NADPH

tetrahydrodictyopterin + NADP+
-
-
-
-
?
1'-oxo-2'-D-hydroxypropyl-tetrahydropterin + NADPH
tetrahydrodictyopterin + NADP+
-
-
-
-
?
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+

L-sepiapterin + NADPH
-
-
-
-
?
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADP+
L-sepiapterin + NADPH
-
-
-
-
?
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADPH

L-erythro-tetrahydrobiopterin + NADP+
-
-
-
-
?
1'-oxo-2'-L-hydroxypropyl-tetrahydropterin + NADPH
L-erythro-tetrahydrobiopterin + NADP+
-
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH

6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
equilibrium lies much in favor of dihydrobiopterin formation
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
equilibrium lies much in favor of dihydrobiopterin formation
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
equilibrium lies much in favor of dihydrobiopterin formation
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
catalyzes the complete reduction of pyrovoyltetrahydropterin to tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
terminal step in the biosynthetic pathway for tetrahydrobiopterin
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
-
-
r
6-pyruvoyl tetrahydropterin + NADPH

L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+
-
last step of the de novo tetrahydrobiopterin biosynthesis initiating from GTP
-
-
?
6-pyruvoyl tetrahydropterin + NADPH
L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+
-
last step of the tetrahydrobiopterin biosynthesis
-
-
?
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH

tetrahydrobiopterin + NADP+
-
-
-
-
r
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH
tetrahydrobiopterin + NADP+
-
-
-
-
r
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH
tetrahydrobiopterin + NADP+
-
-
the cofactor for aromatic amino acid hydrolases in the biosynthesis of aromatc amino acids and dopamine
-
r
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH
tetrahydrobiopterin + NADP+
-
-
-
-
r
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+

L-threo-tetrahydrobiopterin + 2 NADP+
-
final step in the synthesis of tetrahydrobiopterin
no formation of the L-erythro isomer
-
?
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+
L-threo-tetrahydrobiopterin + 2 NADP+
final step in the synthesis of tetrahydrobiopterin
no formation of the L-erythro isomer
-
?
sepiapterin + NADPH + H+

7,8-dihydrobiopterin + NADP+
-
-
-
-
r
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
-
r
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
-
r
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
cellular uptake of sepiapterin across the cell membrane results in an efficient accumulation of tetrahydrobiopterin, sepiapterin relocates across the plasma membrane via two distinct paths, the slower path connects to the cytosolic compartment, overview
-
-
r
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
no stereochemic specification in the publication
-
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
-
r
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
i.e. 2-amino-4-hydroxy-6-lactyl-7,8-dihydropteridine, biosynthesis pathway, overview
i.e. 2-amino-4-hydroxy-6-(1',2'-dihydroxypropyl-)7,8-dihydropteridine, which is a substrate for biosynthesis of tetrahydrobiopterin, i.e. 6R-L-erythro-1',2'-dihydroxy-propyl-2-amino-4-hydroxy-5,6,7,8-tetrahydropteridine, an important component for controling the concentration of catecholamines or indoleamines in the brain and other nerve tissues
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
no stereochemic specification in the publication
-
-
?
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
-
?
sepiapterin + NADPH + H+

tetrahydrobiopterin + NADP+
-
no stereochemic specification in the publication
-
?
sepiapterin + NADPH + H+
tetrahydrobiopterin + NADP+
-
-
no stereochemic specification in the publication
-
?
additional information

?
-
-
D-threo-tetrahydrobiopterin is synthesized via 1'-oxo-2'-D-hydroxypropyl-tetrahydropterin in Dictyostelium discoideum strain Ax2
-
-
?
additional information
?
-
-
quantitative in vivo assay and product determination
-
-
?
additional information
?
-
-
tetrahydropteridines in Dictyostelium are associated with mitochondrial function, probably via direct protection against oxidative stress, tetrahydropteridine deficiency impairs mitochondrial function in Dictyostelium discoideum Ax2
-
-
?
additional information
?
-
-
quantitative in vivo assay and product determination
-
-
?
additional information
?
-
-
D-threo-tetrahydrobiopterin is synthesized via 1'-oxo-2'-D-hydroxypropyl-tetrahydropterin in Dictyostelium discoideum strain Ax2
-
-
?
additional information
?
-
-
final step in the synthesis of tetrahydrobiopterin
-
-
?
additional information
?
-
-
the enzyme is involved in the tetrahydrobiopterin biosynthesis
-
-
?
additional information
?
-
-
sepiapterin or dihydrobiopterin are the precursors of the tetrahydrobiopterin-salvage pathway, enzyme activity and pathway regulation in vivo, overview
-
-
?
additional information
?
-
-
the enzyme catalyzes the final step in the tetrahydrobiopterin biosynthesis
-
-
?
additional information
?
-
-
the enzyme catalyzes the final step in the tetrahydrobiopterin biosynthesis, sepiapterin reductase expression is increased in Parkinsons disease brain tissue
-
-
?
additional information
?
-
native ornithine decarboxylase, EC 4.1.1.17, and sepiapterin reductase physically interact, in silico protein-protein docking simulations
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?
additional information
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native ornithine decarboxylase, EC 4.1.1.17, and sepiapterin reductase physically interact, in silico protein-protein docking simulations
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?
additional information
?
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physical interaction between key polyamines biosynthesis enzyme ornithine decarboxylase (ODC) and sepiapterin reductase (SPR)
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?
additional information
?
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physical interaction between key polyamines biosynthesis enzyme ornithine decarboxylase (ODC) and sepiapterin reductase (SPR)
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?
additional information
?
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final step in the synthesis of tetrahydrobiopterin
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additional information
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important in the metabolism of tetrahydrobiopterin and sepiapterin
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?
additional information
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final step in the synthesis of tetrahydrobiopterin
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?
additional information
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the enzyme might be involved in regulation of biosynthesis of catecholamines, indoleamines, and tetrahydrobiopterins
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additional information
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the enzyme might be involved in regulation of biosynthesis of catecholamines, indoleamines, and tetrahydrobiopterins
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1-norepinephrine
-
3 mM: 30% inhibition
6,7-dimethyldihydropterin
-
0.05 mM: 70% inhibition
6,7-dimethyllumazine
-
slight
6,7-dimethylpterin
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0.05 mM: 46% inhibition; 46% inhibition of the reductive reaction at 0.05 mM
6,7-dimethylpterin-H2
-
70% inhibition of the reductive reaction at 0.05 mM
6-Hydroxymethylpterin
-
0.05 mM: 23% inhibition; 23% inhibition of the reductive reaction at 0.05 mM
6-methyldihydropterin
-
0.05 mM: 59% inhibition
6-methylpterin
-
10% inhibition of the reductive reaction at 0.05 mM; slight
6-Methylpterin-H2
-
59% inhibition of the reductive reaction at 0.05 mM
7-Methylpterin
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0.05 mM: 18% inhibition; 18% inhibition of the reductive reaction at 0.05 mM
9,10-phenanthrenequinone
-
-
alpha-difluoromethylornithine
DFMO, IC50 value of 4.07 mM for SK-N-Be(2)c and 5.79 mM for LAN-5 cells
alpha-difluoromethylornitine
the combination of sulfasalazine with alpha-difluoromethylornitine produces synergistic antiproliferative effects on neuroblastoma cells in vitro
alpha-ketoglutarate
-
slight, IC50 5 mM
benzoic acid
-
0.03 mM: 40% inhibition; 40% inhibition of the reductive reaction at 0.03 mM
biopterin
-
0.05 mM: 24% inhibition; 24% inhibition of the reductive reaction at 0.05 mM
biopterin-H2
-
31% inhibition of the reductive reaction at 0.05 mM
Cibacron Blue 3G-A
-
IC50 0.01 mM
D,L-Lactic acid
-
0.03 mM, 20% inhibition
D-erythro-neopterin
-
14% inhibition of the reductive reaction at 0.05 mM
D-erythro-neopterin-H2
-
7% inhibition of the reductive reaction at 0.05 mM
dihydroxanthopterin
-
slight
DL-lactic acid
-
20% inhibition of the reductive reaction at 0.03 mM
dopamine
-
slight; slight inhibition
Ethacrynic acid
-
IC50 0.03 mM
Fe3+
-
1 mM inhibits entirely
iodoacetate
-
72% inhibition of the reductive reaction at 0.1 mM
Isosepiapterin
-
83% inhibition of the reductive reaction at 0.05 mM; IC50 0.0065 mM, 0.05 mM: 83% inhibition
Isoxanthopterin
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0.05 mM: very slight
KCN
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25% inhibition of the reductive reaction at 2.5 mM; slight
L-erythro-dihydroneopterin
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0.05 mM: 29% inhibition, D-isomer: slight inhibition
L-Erythro-neopterin
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0.05 mM: 21% inhibition, D-isomer: slight inhibition; 21% inhibition of the reductive reaction at 0.05 mM
L-Erythro-neopterin-H2
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29% inhibition of the reductive reaction at 0.05 mM
Leucopterin
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0.05 mM: 21% inhibition; 21% inhibition of the reductive reaction at 0.05 mM
N-acetyl-L-noradrenaline
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weak inhibitor, N-acetyl-L-adrenaline does not bind enzyme
N-acetyl-p-tyramine
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weak, N-acetyl-m-tyramine shows strong inhibition
N-acetyl-serotonin
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complete inhibition
N-acetylphenethylamine
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weak
N-acetyltryptamine
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slight, N-acetyltryptophan does not bind enzyme
n-butyric acid
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IC50 0.032 mM, pH 6.5
N-chloroacetyldopamine
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strong inhibitor
N-chloroacetylserotonin
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strong competitive inhibitor
N-chloroserotonin
strong inhibitor
N-lactoyldopamine
-
potent inhibitor
N-pyruvoyldopamine
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potent inhibitor
n-valeric acid
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IC50 0.25 mM, pH 6.5
NADP+
-
competitive inhibition at fixed concentration with varied NADPH, noncompetitive with varied sepiapterin
NaN3
-
15% inhibition of the reductive reaction at 5 mM; slight
NEM
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35% inhibition of the reductive reaction at 0.1 mM, 88% at 1 mM
p-chloromercuribenzoate
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-
p-mercuribenzoate
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0.1 mM, 93% inhibition
PCMB
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93% inhibition of the reductive reaction at 0.01 mM
Phenobarbital
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slight, IC50 0.6 mM
Propionamide
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0.03 mM, 14% inhibition; 14% inhibition of the reductive reaction at 0.03 mM
pterin
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0.05 mM: 18% inhibition; 18% inhibition of the reductive reaction at 0.05 mM
pyrazole
-
slight, IC50 1 mM
Pyruvic acid
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9% inhibition of the reductive reaction at 0.03 mM; slight
Sn2+
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1 mM inhibits entirely
Xanthopterin-B2
-
12% inhibition of the reductive reaction at 0.05 mM
xanthopterin-H2
-
9% inhibition of the reductive reaction at 0.05 mM
2,4-dinitrophenol

-
37% inhibition of the reductive reaction at 0.01 mM; slight
2,4-dinitrophenol
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0.1 mM: 89% inhibition
6-Carboxypterin

-
77% inhibition of the reductive reaction at 0.05 mM; IC50 0.014 mM, 0.05 mM: 77% inhibition
6-Carboxypterin
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IC50 0.03 mM
acetic acid

-
buffers that contain acetate inhibit activity
acetic acid
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IC50 0.45 mM, pH 6.5
Cd2+

-
1 mM inhibits entirely
Chlorpropamide

noncompetitive; noncompetitive inhibition in sepiapterin reduction and redox cycling
dicoumarol

-
-
dicoumarol
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IC50 0.0006 mM
dihydrobiopterin

-
0.05 mM: 31% inhibition
dihydrobiopterin
-
non-competitive with NADPH or sepiapterin as varying substrate
glibenclamide

noncompetitive inhibition in sepiapterin reduction and redox cycling
indomethacin

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-
indomethacin
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IC50 0.06 mM
Melatonin

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-
Melatonin
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3 mM, almost complete inhibition; strong inhibition
N-acetyldopamine

-
potent competitive inhibitor
N-acetyldopamine
-
very slight
N-acetyldopamine
-
noncompetitive
N-acetylserotonin

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-
N-acetylserotonin
-
potent competitive inhibitor
N-acetylserotonin
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very slight
N-acetylserotonin
-
noncompetitive
N-acetylserotonin
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0.05 mM, 90% inhibition of enzyme from stimulated mononuclear blood cells
N-acetylserotonin
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activity is completely inhibited by the addition of 0.5 mM N-acetylserotonin
N-acetylserotonin
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activity is completely inhibited by the addition of 0.5 mM N-acetylserotonin
N-acetylserotonin
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IC50 0.0006 mM
N-acetylserotonin
-
3 mM, complete inhibition
N-methoxyacetylserotonin

-
strong competitive inhibitor
N-methoxyacetylserotonin
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noncompetitive
N-methoxyacetylserotonin
strong competitive inhibitor
NADPH

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more than 0.05 mM causes substrate inhibition
NADPH
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product inhibition, with more than 0.1 mM, with 0.05 mM sepiapterin
propionic acid

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43% inhibition of the reductive reaction at 0.03 mM; IC50 0.0045 mM, pH 6.5
propionic acid
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IC50 0.3 mM, pH 6.4
sepiapterin

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above 0.05 mM
sepiapterin
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product inhibition, with more than 0.05 mM, with 0.1 mM NADPH
sulfamethoxazole

noncompetitive; noncompetitive inhibition in sepiapterin reduction and redox cycling
sulfapyridine

noncompetitive; noncompetitive inhibition in sepiapterin reduction and redox cycling
sulfasalazine

i.e. 5-((4-(2-pyridylsulfamoyl) phenyl)azo) salicylic acid or 2-hydroxy-5-[(E)-2-{4-[(pyridin-2-yl)sulfamoyl]phenyl}diazen-1-yl]benzoic acid or SSZ, an FDA-approved salicylate-based anti-inflammatory and immuno-modulatory drug, inhibits sepiapterin reductase. Computational docking of inhibitor sulfasalazine into sepiapterin reductase. The receptor pocket comprises residues Gly11, Ser13, Arg14, Phe16 (region 1), Ala38, Arg39 (region 2), Asn97, Ala98, Gly99, Ser100 (region 3), Tyr167 (region 4), and Leu198, Thr200, Met202 (region 5). Sulfasalazine exhibits an IC50 value of 0.133 mM for SK-N-Be(2)c and 0.337 mM for LAN-5 cells; inhibits the growth of neuroblastoma cells in vitro, presumably due to the inhibition of sepiapterin reductase as predicted by computational docking of sulfasalazine into sepiapterin reductase. The combination of sulfasalazine with alpha-difluoromethylornitine produces synergistic antiproliferative effects in vitro
sulfasalazine
noncompetitive; noncompetitive inhibition in sepiapterin reduction and redox cycling
sulfathiazole

noncompetitive; noncompetitive inhibition in sepiapterin reduction and redox cycling
Tolbutamide

noncompetitive inhibition in sepiapterin reduction and redox cycling
additional information

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Li+, Na+, Co2+, Mn2+, Ni2+ show slight inhibition at 1 mM
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additional information
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other unconjugated pteridines, e.g. xanthopterin, that cause slight inhibition of enzymatic reduction
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additional information
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the enzyme activity is not affected by laminar shear stress in endothelial cells, overview
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additional information
synergism of sulfasalazine and alpha-difluoromethylornithine (DFMO) combination treatment in neuroblastoma cells
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additional information
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synergism of sulfasalazine and alpha-difluoromethylornithine (DFMO) combination treatment in neuroblastoma cells
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additional information
sulfonylurea- or sulfonamide-based drugs inhibit sepiapterin reduction and chemical redox cycling by sepiapterin reductase
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additional information
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sulfonylurea- or sulfonamide-based drugs inhibit sepiapterin reduction and chemical redox cycling by sepiapterin reductase
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additional information
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no inhibition by 1-epinephrine, phenylalanine, tyrosine, L-DOPA, L-tryptophan, L-5-hydroxy-tryptophan
-
additional information
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inhibition by specific antibody from rabbits
-
additional information
sulfonylurea- or sulfonamide-based drugs inhibit sepiapterin reduction and chemical redox cycling by sepiapterin reductase
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