Information on EC 1.1.1.147 - 16alpha-hydroxysteroid dehydrogenase

for references in articles please use BRENDA:EC1.1.1.147

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

EC Tree 1 Oxidoreductases 1.1 Acting on the CH-OH group of donors 1.1.1 With NAD⁺ or NADP⁺ as acceptor 1.1.1.147 16alpha-hydroxysteroid dehydrogenase

The enzyme appears in viruses and cellular organisms

Reaction Schemes

hide

show

a 16alpha-hydroxysteroid

Synonyms
16alpha-hydroxy steroid dehydrogenase, more

top print hide

show all columns Go to Synonym Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

16alpha-hydroxy steroid dehydrogenase

top print hide

show all columns Go to Reaction Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

a 16alpha-hydroxysteroid + NAD(P)+ = a 16-oxosteroid + NAD(P)H + H+

top print hide

show all columns Go to Reaction Type Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

REACTION TYPE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

oxidation

redox reaction

reduction

top print hide

Go to Systematic Name Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SYSTEMATIC NAME

IUBMB Comments

16alpha-hydroxysteroid:NAD(P)+ 16-oxidoreductase

top print hide

show all columns Go to CAS Registry Number Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CAS REGISTRY NUMBER

COMMENTARY

37250-74-1

top print hide

show all columns Go to Substrate/Product Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

Reversibility
r=reversible
ir=irreversible
?=not specified

16-ketotestosterone + NAD(P)H

16alpha-hydroxytestosterone + NAD(P)+

Rattus sp.

285968

Substrates: -
Products: -

16-oxo-17beta-estradiol + NADH + H+

16alpha-hydroxy-17beta-estradiol + NAD+

Rattus sp.

285968

Substrates: -
Products: -

17alpha-estradiol-16-one + NAD(P)H

16alpha-hydroxy-17alpha-estradiol + NAD(P)+

Rattus sp.

285968

Substrates: best substrate tested, changing configuration or oxidation state of the neighboring oxygen substituent at C-17 increases the rate of reduction
Products: -

17beta-estradiol-16-one + NAD(P)H

16alpha-hydroxy-17beta-estradiol + NAD(P)+

2 entries

3-hydroxy-estra-1,3,5(10)-triene-16,17-dione + NAD(P)H + H+

3,16alpha-dihydroxy-estra-1,3,5(10)-triene-17-one + NAD(P)+

Rattus sp.

285968

Substrates: -
Products: -

3beta,17beta-dihydroxy-androst-5-en-16-one + NAD(P)H

3beta,16alpha,17beta-trihydroxyandrost-5-ene + NAD(P)+

Rattus sp.

285968

Substrates: low activity
Products: -

estra-1,3,5(10)-triene-3,16beta,17beta-triol + NAD(P)+

estra-1,3,5(10)-triene-3,17beta-diol-16-one + NAD(P)H

Rattus sp.

285968

Substrates: -
Products: -

estra-1,3,5(10)triene-3,17-beta-diol-16-one + NAD(P)H

estra-1,3,5(10)triene-3,16alpha,17-triol + NAD(P)+

Rattus sp.

285968

Substrates: low activity
Products: -

17beta-estradiol-16-one + NAD(P)H

16alpha-hydroxy-17beta-estradiol + NAD(P)+

Homo sapiens

285969

Substrates: -
Products: -

17beta-estradiol-16-one + NAD(P)H

16alpha-hydroxy-17beta-estradiol + NAD(P)+

Rattus sp.

285968

Substrates: -
Products: -

top print hide

show all columns Go to Cofactor Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

NADH

Rattus sp.

40% lower reaction rate than NADPH

285968

NADPH

Rattus sp.

40% greater reaction rate than NADH

285968

top print hide

show all columns Go to Inhibitor Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1,10-phenanthroline

Rattus sp.

complete inhibition at 0.1 mM

285968

2,2'-bipyridine

Rattus sp.

19% inhibition at 0.1 mM

285968

2-Iodoacetamide

Rattus sp.

86% inhibition at 1 mM

285968

8-hydroxy-5-quinolinesulfonate

Rattus sp.

89% inhibition at 1 mM

285968

Ag+

Rattus sp.

78% inhibition at 0.01 mM

285968

Cu2+

Rattus sp.

98% inhibition at 0.01 mM

285968

EDTA

Rattus sp.

97% inhibition at 0.01 mM

285968

N-ethylmaleimide

Rattus sp.

94% inhibition at 0.1 mM

285968

o-Iodosobenzoate

Rattus sp.

complete inhibition at 0.1 mM

285968

p-hydroxymercuribenzoate

Rattus sp.

complete inhibition at 0.01 mM

285968

Zn2+

Rattus sp.

84% inhibition at 0.1 mM

285968

top print hide

show all columns Go to KM Value Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.28

16-ketotestosterone

Rattus sp.

285968

0.07

17alpha-estradiol-16-one

Rattus sp.

285968

0.089

17beta-estradiol-16-one

Rattus sp.

285968

0.083

NADH

Rattus sp.

285968

0.041

NADPH

Rattus sp.

285968

top print hide

show all columns Go to pH Optimum Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

6.1 - 6.2

Rattus sp.

285968

top print hide

show all columns Go to Organism Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

brenda

brenda

show all columns Go to Source Tissue Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

kidney

Rattus sp.

inducible by estrogen administration

brenda

brenda

Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

top print hide

Go to General Stability Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

extremely unstable, glycerol partially protects against inactivation

Rattus sp.

285968

top print hide

Go to Purification (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

partial

Rattus sp.

285968

top print hide References Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

285968

Meigs, R.A.; Ryan, K.J.

16alpha-Hydroxysteroid dehydrogenase of rat kidney. Purification, assay, and properties

J. Biol. Chem.

241

4011-4015

1966

Rattus sp.

4380686

brenda

285969

Preumont, P.; Smuk, M.

16-Dehydrogenase in human placenta

Acta Endocrinol.

760-765

1975

Homo sapiens

1173964

brenda

Entries 1 - 20 of 2

Show complete table

top print hide 10 entries

EXTERNAL LINKS (specific for EC-Number 1.1.1.147)

ExplorEnz (official portal for IUBMB Enzyme Nomenclature)

Expasy Enzyme Nomenclature Database

KEGG

SABIO-RK

NCBI: PubMed, Protein, Nucleotide, Structure, Gene, OMIM

Enzyme Nomenclature (alternative site)

UniProt

PDB

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)

html completed