Information on EC 1.1.1.127 - 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase

for references in articles please use BRENDA:EC1.1.1.127
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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
1.1.1.127
-
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RECOMMENDED NAME
GeneOntology No.
2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-dehydro-3-deoxy-D-gluconate + NAD+ = (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH + H+
show the reaction diagram
The enzyme from Pseudomas acts equally well on NAD+ or NADP+, while that from Erwinia chrysanthemi and E. coli is more specific for NAD+
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
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redox reaction
-
-
-
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reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3,6-anhydro-alpha-L-galactopyranose degradation
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-
4-deoxy-L-threo-hex-4-enopyranuronate degradation
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Pentose and glucuronate interconversions
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SYSTEMATIC NAME
IUBMB Comments
2-dehydro-3-deoxy-D-gluconate:NAD+ 5-oxidoreductase
The enzyme from Pseudomonas acts equally well on NAD+ or NADP+, while that from Erwinia chrysanthemi and Escherichia coli is more specific for NAD+.
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-56-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,5-didehydro-3-deoxy-D-gluconate + NADH + H+
2-dehydro-3-deoxy-D-gluconate + NAD+
show the reaction diagram
A0A093RP61
-
-
-
?
2,5-didehydro-3-deoxy-D-gluconate + NADPH + H+
2-dehydro-3-deoxy-D-gluconate + NADP+
show the reaction diagram
A0A093RP61
-
-
-
?
2-dehydro-3-deoxy-D-gluconate + NAD+
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
show the reaction diagram
pyruvate + NAD+
? + NADH
show the reaction diagram
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10% of the activity compared to 2-dehydro-3-deoxy-D-gluconate
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,5-didehydro-3-deoxy-D-gluconate + NADH + H+
2-dehydro-3-deoxy-D-gluconate + NAD+
show the reaction diagram
A0A093RP61
-
-
-
?
2,5-didehydro-3-deoxy-D-gluconate + NADPH + H+
2-dehydro-3-deoxy-D-gluconate + NADP+
show the reaction diagram
A0A093RP61
-
-
-
?
2-dehydro-3-deoxy-D-gluconate + NAD+
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
NADPH
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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20% activation at 1 mM
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
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72% inhibition at 0.01 mM
ATP
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30% inhibition for both reduction and oxidation reaction at 1 mM
p-chloromercuribenzoate
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50% inhibition at 0.02 mM
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.34
(4S)-4,6-dihydroxy-2,5-dioxohexanoate
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7.7
2-dehydro-3-deoxy-D-gluconate
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0.0021 - 0.03
NADH
0.005
NADPH
A0A093RP61
at pH 7.2 and 45°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.024
NADH
A0A093RP61
at pH 7.2 and 45°C
0.021
NADPH
A0A093RP61
at pH 7.2 and 45°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
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reduction reaction
10
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oxidation reaction
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
A0A093RP61
4 * 25000, SDS-PAGE
90000
A0A093RP61
gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
A0A093RP61
4 * 25000, SDS-PAGE
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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in complex with NAD+, hanging drop vapor diffusion method, using 5% (w/v) polyethylene glycol 4000, and 10% (v/v) isopropanol
A0A093RP61
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
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stable for 15 h
40
A0A093RP61
the enzyme shows more than 70% activity at 40°C for 5 min
46
-
half-life: 10 min, NADH increases thermal stability
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
1 mM NAD+ or NADP+ increase stability
-
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 2 mM DTT, 6 months
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0-3°C, 1 mM NAD+ or NADP+, 50-67% retaining activity after 5 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, 2step chromatography
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Toyopearl DEAE-650 M column chromatography, Toyopearl butyl-650 M column chromatography, Q-Sepharose column chromatography, and Superdex 200 pg gel filtration
A0A093RP61
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
A0A093RP61
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Show AA Sequence (660 entries)
Please use the Sequence Search for a specific query.
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LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.127)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)