We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The enzyme from Pseudomonas acts equally well on NAD+ or NADP+, while that from Erwinia chrysanthemi and Escherichia coli is more specific for NAD+.
The enzyme appears in viruses and cellular organisms
Synonyms
2-dehydro-3-deoxy-d-gluconate 5-dehydrogenase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
-
-
2-keto-3-deoxy-D-gluconate (3-deoxy-D-glycero-2,5-hexodiulosonic acid) dehydrogenase
-
-
-
2-keto-3-deoxy-D-gluconate dehydrogenase
2-keto-3-deoxygluconate (nicotinamide adenine dinucleotide (phosphate)) dehydrogenase
-
-
-
2-keto-3-deoxygluconate 5-dehydrogenase
-
-
-
2-keto-3-deoxy-D-gluconate dehydrogenase
-
-
-
2-keto-3-deoxy-D-gluconate dehydrogenase
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-dehydro-3-deoxy-D-gluconate + NAD+ = (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH + H+
The enzyme from Pseudomas acts equally well on NAD+ or NADP+, while that from Erwinia chrysanthemi and E. coli is more specific for NAD+
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-dehydro-3-deoxy-D-gluconate:NAD+ 5-oxidoreductase
The enzyme from Pseudomonas acts equally well on NAD+ or NADP+, while that from Erwinia chrysanthemi and Escherichia coli is more specific for NAD+.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,5-didehydro-3-deoxy-D-gluconate + NADH + H+
2-dehydro-3-deoxy-D-gluconate + NAD+
-
-
-
?
2,5-didehydro-3-deoxy-D-gluconate + NADPH + H+
2-dehydro-3-deoxy-D-gluconate + NADP+
-
-
-
?
2-dehydro-3-deoxy-D-gluconate + NAD+
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
pyruvate + NAD+
? + NADH
-
10% of the activity compared to 2-dehydro-3-deoxy-D-gluconate
-
?
2-dehydro-3-deoxy-D-gluconate + NAD+
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
involved in pectinolysis by some phytopathogenic bacteria
-
r
2-dehydro-3-deoxy-D-gluconate + NAD+
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
irreversible at pH 7.0
-
ir
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,5-didehydro-3-deoxy-D-gluconate + NADH + H+
2-dehydro-3-deoxy-D-gluconate + NAD+
-
-
-
-
?
2,5-didehydro-3-deoxy-D-gluconate + NADPH + H+
2-dehydro-3-deoxy-D-gluconate + NADP+
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate + NAD+
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
2-dehydro-3-deoxy-D-gluconate + NAD+
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
involved in pectinolysis by some phytopathogenic bacteria
-
r
2-dehydro-3-deoxy-D-gluconate + NAD+
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
irreversible at pH 7.0
-
ir
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NAD+
-
-
NADH
-
-
NADH
-
the enzyme utilizes both NADH and NADPH
NADP+
-
-
NADP+
-
53% lower activity than with NAD+
NADPH
-
62% lower activity than with NADH
NADPH
-
the enzyme utilizes both NADH and NADPH
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ca2+
-
20% activation at 1 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ag+
-
72% inhibition at 0.01 mM
ATP
-
30% inhibition for both reduction and oxidation reaction at 1 mM
p-chloromercuribenzoate
-
50% inhibition at 0.02 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.34
(4S)-4,6-dihydroxy-2,5-dioxohexanoate
-
-
7.7
2-dehydro-3-deoxy-D-gluconate
-
-
0.005
NADPH
-
at pH 7.2 and 45°C
0.0021
NADH
-
at pH 7.2 and 45°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.024
NADH
-
at pH 7.2 and 45°C
0.021
NADPH
-
at pH 7.2 and 45°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7 - 7.5
-
reduction reaction
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
45
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
25000
-
4 * 25000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
homotetramer
-
4 * 25000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
in complex with NAD+, hanging drop vapor diffusion method, using 5% (w/v) polyethylene glycol 4000, and 10% (v/v) isopropanol
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
40
-
the enzyme shows more than 70% activity at 40°C for 5 min
46
-
half-life: 10 min, NADH increases thermal stability
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1 mM NAD+ or NADP+ increase stability
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-20°C, 2 mM DTT, 6 months
-
0-3°C, 1 mM NAD+ or NADP+, 50-67% retaining activity after 5 weeks
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
to homogeneity, 2step chromatography
-
Toyopearl DEAE-650 M column chromatography, Toyopearl butyl-650 M column chromatography, Q-Sepharose column chromatography, and Superdex 200 pg gel filtration
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli BL21(DE3) cells
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Condemine, G.; Hugouvieux-Cotte-Pattat, N.; Robert-Baudouy, J.
An enzyme in the pectinolytic pathway of Erwinia chrysanthemi: 2-Keto-3-deoxygluconate oxidoreductase
J. Gen. Microbiol.
130
2839-2844
1984
Dickeya chrysanthemi
-
brenda
Preiss, J.; Ashwell, G.
Polygalacturonic acid metabolism in bacteria. II. Formation and metabolism of 3-deoxy-D-glycero-2,5-hexodiulosonic acid
J. Biol. Chem.
238
1577-1583
1963
Pseudomonas sp.
brenda
Takase, R.; Maruyama, Y.; Oiki, S.; Mikami, B.; Murata, K.; Hashimoto, W.
Structural determinants in bacterial 2-keto-3-deoxy-D-gluconate dehydrogenase KduD for dual-coenzyme specificity
Proteins
84
934-947
2016
Pectobacterium carotovorum subsp. carotovorum
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
1.1.1.127 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
more
top
Information
