Information on EC 1.1.1.118 - glucose 1-dehydrogenase (NAD+)

additional information

Substrates: no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+
Products: -

additional information

Saccharolobus solfataricus MT-4 / DSM 5833

Substrates: no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+
Products: -

additional information

AHI17928.1

Substrates: the enzyme shows no activity towards D-xylose, D-mannose, D-maltose, glucose 6-phosphate and sucrose
Products: -

show all columns Go to Natural Substrate Search

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

D-glucose + NAD+

D-glucono-1,5-lactone + NADH + H+

3 entries

D-glucose + NAD+

D-glucono-1,5-lactone + NADH + H+

739713

Substrates: -
Products: -

D-glucose + NAD+

D-glucono-1,5-lactone + NADH + H+

739713

Substrates: -
Products: -

D-glucose + NAD+

D-glucono-1,5-lactone + NADH + H+

Substrates: -
Products: -

show all columns Go to Cofactor Search

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

NAD+

NADP+

NAD+

NAD+

NAD+

NAD+

cofactor

NAD+

cofactor

NAD+

AHI17928.1

the enzyme prefers NADP+ as coenzyme to NAD+

NADP+

NADP+

AHI17928.1

the enzyme prefers NADP+ as coenzyme to NAD+

show all columns Go to Metals and Ions Search

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Ca2+

maximally active at 20 mM

Mg2+

maximally active at 20 mM

Mn2+

maximally active at 20 mM

Zn2+

AHI17928.1

additional information

Zn2+ is partially effective, whereas Ni2+, Cd2+ and univalent cations are ineffective in promoting enzyme reactivation

show all columns Go to Inhibitor Search

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2-mercaptoethanol

rapid inactivation at 1 mM

5,5'-dithiobis-(2-nitrobenzoic acid)

Cu2+

AHI17928.1

EDTA

Guanidinium chloride

rapid inactivation at 4 M

N-ethylmaleimide

NADPH

inhibits the NAD+-dependent carbohydrate oxidations in a competitive manner with respect to NAD+

tetrahydrofuran

additional information

D-alactose, D-mannose and D-ribose, in concentrations as high as 0.04 mM do not inhibit glucose oxidation by NAD+

EDTA

5% residual activity at 50 mM, addition of Mg2+ in excess restored the initial activity

EDTA

AHI17928.1

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DISEASE

TITLE OF PUBLICATION

LINK TO PUBMED

Acrodynia

Identification and characterization of a Pantoea citrea gene encoding glucose dehydrogenase that is essential for causing pink disease of pineapple.

8979341

Dehydration

Metabolic pathway promiscuity in the archaeon Sulfolobus solfataricus revealed by studies on glucose dehydrogenase and 2-keto-3-deoxygluconate aldolase.

12824170

Dehydration

The structural basis for substrate promiscuity in 2-keto-3-deoxygluconate aldolase from the Entner-Doudoroff pathway in Sulfolobus solfataricus.

15265860

Galactosemias

Erroneous glucose recordings while using mutant variant of quinoprotein glucose dehydrogenase glucometer in a child with galactosemia.

24251189

glucose 1-dehydrogenase (nad+) deficiency

[Phosphate glucose dehydrogenase deficiency causing hyperbilirubinemia in the newborn]

5789870

Malaria

Analysis of the genetic variants of glucose-6-phosphate dehydrogenase in inhabitants of the 4th Nile cataract region in Sudan.

23146719

Pancreatitis

Peritoneal dialysis: a primary care perspective.

16809653

Photosensitivity Disorders

Biohydrogen production from sucrose using the visible light sensitization of artificial Zn chlorophyll-a.

12526718

Photosensitivity Disorders

Photoinduced biohydrogen production from biomass.

19325796

Photosensitivity Disorders

Visible light induced biohydrogen production from sucrose using the photosensitization of Mg chlorophyll-a.

12121148

Photosensitivity Disorders

Visible light-induced H2 production from cellulose using photosensitization of Mg chlorophyll a.

100149770

Starvation

Glucose dehydrogenase, glucose-6-phosphate dehydrogenase and hexokinase in liver of rainbow trout (Salmo gairdneri). Effects of starvation and temperature variations.

1769217

Starvation

[Variations of enzymatic activities in an asporogenic mutant of Bacillus megaterium for various nutritional deficiencies]

826332

show all columns Go to KM Value Search

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

5.8

D-fucose

D-galactose

in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

0.44 - 8

D-glucose

2.2 - 68

D-xylose

2 entries

0.67 - 80

NAD+

0.03 - 157.9

NADP+

0.44

D-glucose

in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

0.86

D-glucose

7.48

D-glucose

AHI17928.1

pH 7.5, 40°C

D-glucose

in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

2.2

D-xylose

in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

D-xylose

in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

0.67

NAD+

AHI17928.1

pH 7.5, 40°C

1.2

NAD+

in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

1.2

NAD+

in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

NAD+

0.03

NADP+

in the presence of D-galactose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

0.03

NADP+

in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

0.03

NADP+

in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C

157.9

NADP+

AHI17928.1

pH 7.5, 40°C

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

64.9

D-glucose

AHI17928.1

pH 7.5, 40°C

69.7

NAD+

AHI17928.1

pH 7.5, 40°C

101.9

NADP+

AHI17928.1

pH 7.5, 40°C

show all columns Go to kcat/KM Value Search

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

8.67

D-glucose

AHI17928.1

pH 7.5, 40°C

104

NAD+

AHI17928.1

pH 7.5, 40°C

0.64

NADP+

AHI17928.1

pH 7.5, 40°C

show all columns Go to Ki Value Search

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Ki VALUE [mM]

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.075

NADPH

using D-glucose as a substrate

show all columns Go to IC50 Value Search

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IC50 VALUE [mM]

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

5,5'-dithiobis-(2-nitrobenzoic acid)

Saccharolobus solfataricus

at 25°C and pH 9

N-ethylmaleimide

Saccharolobus solfataricus

at 25°C and pH 9

show all columns Go to Specific Activity Search

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.7

unpurified enzyme, at 70°C

437

after 623fold purification, at 70°C

8.5

AHI17928.1

40°C, pH 7.5, coenzyme: NAD+

86.3

AHI17928.1

40°C, pH 7.5, coenzyme: NADP+

show all columns Go to pH Optimum Search

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

10 - 10.2

Corynebacterium sp.

285777

assay at

7.5

AHI17928.1

show all columns Go to pH Range Search

8 - 8.5

Tris-HCl buffer

9 - 10

glycine-NaOH buffer

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pH RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

5.5 - 8

AHI17928.1

pH 5.5: about 50% of maximal activity, pH 8.0: about 40% of maximal activity

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TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

assay at

AHI17928.1

show all columns Go to Temperature Range Search

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TEMPERATURE RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

10 - 60

AHI17928.1

10°C: about 35% of maximal activity, 20°C: about 80% of maximal activity, 60°C: about 75% of maximal activity

show all columns Go to Organism Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

701045, 739713

brenda

Corynebacterium sp. No. 93-1

701045, 739713

brenda

Corynebacterium sp.

No. 93-1

285777

brenda

No. 93-1

285777

brenda

i.e. Acetobacter xylinum

Pseudomonas putida IAM 12014

brenda

Pseudomonas putida

IAM 12014

285779

brenda

IAM 12014

285779

brenda

2 entries

Pseudomonas sp. MSU-1

MSU-1

brenda

brenda

Saccharolobus solfataricus MT-4 / DSM 5833

brenda

a codon-optimized gene Sygdh encoding glucose 1-dehydrogenase (SyGDH) is synthesized based on the putative GDH gene sequence (Ta0897) in Thermoplasma acidophilum genomic DNA, and expressed in Escherichia coli BL21(DE3)

AHI17928.1

GenBank

brenda

brenda

MSU-1

brenda

Highest Expressing Human Cell Lines

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Cell Line Links

Gene Links

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

Q9HK51 pBLAST

Q9HK51_THEAC

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

255

27873

TrEMBL

Show Sequence

other Location (Reliability: 5)

Q97CM7 pBLAST

Q97CM7_THEVO

Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)

261

28195

TrEMBL

Show Sequence

Go to PDB and Structure Links Search

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PDB

SCOP

CATH

UNIPROT

ORGANISM

Thermoplasma volcanium GSS1

3vtz, download, 3D-view

SCOPe (3vtz)

CATH (3vtz)

Q97CM7

2dtd, download, 3D-view

SCOPe (2dtd)

CATH (2dtd)

2dte, download, 3D-view

SCOPe (2dte)

CATH (2dte)

2dtx, download, 3D-view

SCOPe (2dtx)

CATH (2dtx)

2zk7, download, 3D-view

SCOPe (2zk7)

CATH (2zk7)

show all columns Go to Molecular Weight Search

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

124000

gel filtration

30000

x * 30000, SDS-PAGE

739713

30100

4 * 30100, gel filtration, in the presence of 5% (w/v) SDS

60200

2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride

show all columns Go to Subunit Search

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

homodimer

homotetramer

x * 30000, SDS-PAGE

x * 30000, SDS-PAGE

homodimer

2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride

homodimer

Saccharolobus solfataricus MT-4 / DSM 5833

2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride

homotetramer

4 * 30100, gel filtration, in the presence of 5% (w/v) SDS

homotetramer

Saccharolobus solfataricus MT-4 / DSM 5833

4 * 30100, gel filtration, in the presence of 5% (w/v) SDS

show all columns Go to Protein Variants Search

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

E170K

has enhanced stability when compared to the wild-type with half-life of ca. 9 min at 65°C. Stability dependence on salt concentration

E170K/Q252L

has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95

P45A/F155Y/V227A

has enhanced stability when compared to the wild-type with half-life of ca. 0.05 min at 65°C. Most labile variant has significantly lower stability in ethanol, acetone, and 1,4-dioxane than other variants

P45A/N46E/F155Y/E170K/V227A/W230F/Q252L

has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95

E170K

has enhanced stability when compared to the wild-type with half-life of ca. 9 min at 65°C. Stability dependence on salt concentration

E170K/Q252L

has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95

P45A/F155Y/V227A

P45A/N46E/F155Y/E170K/V227A/W230F/Q252L

has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

5 - 9