EC 1.1.1.280: (S)-3-hydroxyacid-ester dehydrogenase

This is an abbreviated version!
For detailed information about (S)-3-hydroxyacid-ester dehydrogenase, go to the full flat file.

Reaction

Synonyms

2-methyl-3-oxobutanoate reductase, 3-oxo ester (S)-reductase, EC 1.2.1.56, reductase, 3-oxo ester (S)-

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.280 (S)-3-hydroxyacid-ester dehydrogenase

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All data fields related to EC 1.1.1.280

Results	in table
2	Application
1	CAS Registry Number
2	Cofactor
8	Inhibitors
6	KM Value [mM]
1	Localization
4	Molecular Weight [Da]
3	Organism
3	pH Optimum
1	pH Stability
2	Purification (Commentary)
1	Reaction
3	Reaction Type
3	Reference
1	Specific Activity [micromol/min/mg]
16	Substrates and Products (Substrate)
3	Subunits
4	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]

Purification

Purification on EC 1.1.1.280 - (S)-3-hydroxyacid-ester dehydrogenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

using ammonium sulfate fractionation, ion exchange chromatography, affinity chromatography, and gel filtration

Klebsiella pneumoniae

-

639094

using streptomycin sulfate treatment, Sephadex G-25 filtration, DEAE-Sepharose CL-6B chromatography, Sephadex G-150 filtration, Sepharose 6B filtration and hydroxyapatite chromatography

Saccharomyces cerevisiae

-

639092