EC Number |
Protein Variants |
Reference |
---|
6.4.1.1 | A465R |
mutation in the alpha subunit, abolishes the formation of the holoenzyme, catalytically inactive |
-, 745859 |
6.4.1.1 | A55T |
mutation in the beta subunit, reduces activity by 50fold and interferes with biotin binding to the active site,mutant exhibits growth on pyruvate similar to the wild type |
745859 |
6.4.1.1 | A610T |
more than 30fold loss in catalytic efficiency |
706790 |
6.4.1.1 | A610T |
naturally occurring mutation involved in pyruvate carboxylase deficiency type A, the mutant's catalytic activity and steady-state level are markedly decreased |
690828 |
6.4.1.1 | A610T |
reduced enzyme activity, no import of the enzyme into mitochondria |
649744 |
6.4.1.1 | C249A |
only small effects on enzyme activity |
650234 |
6.4.1.1 | D1018A |
mutant has increased activity in the absence of acetyl-CoA, but not in its presence |
744903 |
6.4.1.1 | D420A |
mutation affects acetyl-CoA binding, enhances the activity of the enzyme in the absence of acetyl-CoA |
-, 744355 |
6.4.1.1 | D471A |
mutant exhibits no acetyl CoA-activation |
-, 744903 |
6.4.1.1 | D502A/E507A |
mutation in the alpha subunit, mutant forms a stable holoenzyme, about 40% of wild-type activity |
745859 |