EC Number   |
Protein Variants |
Reference |
|---|
    5.4.99.7 | C457D |
less active than the wild-type |
-, 661489 |
| 5.4.99.7 | C457D/A525C |
similar activity as the wild-type |
661489 |
| 5.4.99.7 | C457D/E526A |
decrease in enzyme functionality |
-, 661489 |
| 5.4.99.7 | C457D/E526C |
very sensitive to the thiol-reacting agent dodecylmaleimide, specific activity and thermal stability are severely reduced |
-, 661489 |
| 5.4.99.7 | C457D/E526D |
no effect on catalytic avtivity |
-, 661489 |
| 5.4.99.7 | C457D/E526Q |
no effect on catalytic avtivity |
-, 661489 |
| 5.4.99.7 | C457G |
site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview |
728421 |
| 5.4.99.7 | C457G/T509G |
site-directed mutagenesis, the mutations disrupt the pre-existing H-bond to the protonating Asp456 and the intrinsic His234-Tyr510 H-bond network, respectively, and generates achilleol A as the major product |
728421 |
| 5.4.99.7 | C703D |
site-directed mutagenesis, the mutant shows unaltered product spectrum compared to the wild-type enzyme |
727106 |
| 5.4.99.7 | C703G |
site-directed mutagenesis, the mutant shows altered product spectrum compared to the wild-type enzyme, but not as diverse as mutants C703I and C703H |
727106 |
