EC Number   |
Protein Variants |
Reference |
|---|
   3.2.2.27 | A205S |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
-, 694957 |
| 3.2.2.27 | A214R |
site-directed mutagenesis, the mutant shows altered substrate specificity for cleavage of uracil-DNA base pairs in comparison to the wild-type enzyme, overview |
693682 |
| 3.2.2.27 | A59Y |
the mutation leads to a decrease in its activity on xanthine, and 5-hydroxymethyluracil containing single stranded DNAs but not on uracil containing single stranded DNA |
-, 751741 |
| 3.2.2.27 | C101A |
absence of a fully coordinated [4Fe-4S]2+ cluster, loses considerable activity after incubation in assay buffer for two min, with about 20% of the original level of activity remaining |
721666 |
| 3.2.2.27 | C17S |
the mutant enzyme has a brown color similar to that of the wild type protein |
722659 |
| 3.2.2.27 | C17S/C20S |
colorless mutant enzyme, loss of the Fe-S cluster, decrease in activity |
722659 |
| 3.2.2.27 | C20S |
the mutant enzyme has a brown color similar to that of the wild type protein |
722659 |
| 3.2.2.27 | C85A |
absence of a fully coordinated [4Fe-4S]2+ cluster, loses considerable activity after incubation in assay buffer for two min, with about 20% of the original level of activity remaining |
721666 |
| 3.2.2.27 | D132A |
inactive |
732133 |
| 3.2.2.27 | D145N |
the mutant shows increased class switch recombination efficiency and reduced uracil removal activity compared to the wild type enzyme |
731927 |
