EC Number   |
Protein Variants |
Reference |
|---|
   3.2.1.52 | betaAsp452Asn/betaLeu453Arg |
the mutant enzyme of HexB exhibits more than 30fold increase in its ability to hydrolyze a 6-sulfated substrate and is able to hydrolyze GM2 ganglioside when the GM2 activator protein is replaced by sodium taurocholate |
654853 |
| 3.2.1.52 | D148A |
the mutant shows 868fold decreased catalytic efficiency with 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide compared to the wild type enzyme |
749904 |
| 3.2.1.52 | D148N |
the mutant shows 4190fold decreased catalytic efficiency with 3-fluoro-4-nitrophenyl N-acetyl-beta-D-galactosaminide compared to the wild type enzyme |
749904 |
| 3.2.1.52 | D183N |
no activity with 4-methylumbelliferyl N-acetyl-beta-D-glucosamine. Kcat/KM for 4-nitrophenyl N-acetyl-beta-D-glucosamine is 13333fold lower than wild-type value. Biofilm-detachment activity is very low |
679793 |
| 3.2.1.52 | D246N |
mutant |
393684 |
| 3.2.1.52 | D306A |
the mutant enzyme is inactive with 4-nitrophenyl N-acetyl-beta-D-glucosaminide and chitobiose |
-, 750872 |
| 3.2.1.52 | D306E |
the mutant enzyme shows 4.2% activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide compared to the wild type and is inactive with chitobiose |
-, 750872 |
| 3.2.1.52 | D306E/E307D |
the mutant enzyme shows 2.7% activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide compared to the wild type and is inactive with chitobiose |
750872 |
| 3.2.1.52 | D306N |
the mutant enzyme is inactive with 4-nitrophenyl N-acetyl-beta-D-glucosaminide and chitobiose |
-, 750872 |
| 3.2.1.52 | D321E |
site-directed mutagenesis, the Hex1 mutant shows reduced activity compared to the wild-type enzyme |
709527 |
