EC Number   |
Protein Variants |
Reference |
|---|
   3.1.4.35 | C1128S |
the unprenylated mutant enzyme is mainly localized to the cytosol |
690833 |
| 3.1.4.35 | C68A |
expression of isoform PDE6 wild-type and mutant C68A gamma-subunit in Escherichia coli, preparation of homogeneous and isotopically labeled protein for NMR analysis |
682655 |
| 3.1.4.35 | D166A |
mutation reduces cGMP affinity |
669288 |
| 3.1.4.35 | D169A |
mutant enzyme shows no binding of cGMP |
669288 |
| 3.1.4.35 | D196A |
the mutation disrupts cGMP binding and increases cAMP affinity in constructs containing only GAF (mammalian cGMP-dependent PDEs, Anabaena adenylyl cyclases, and Escherichia coli FhlA), causing an altered cAMP-bound structural conformation |
693110 |
| 3.1.4.35 | D299A |
mutation in GAF-domain, no change in cGMP binding affinity |
651322 |
| 3.1.4.35 | D644A |
10% and 4% of maximal activity in the absence of Mn2+ and Mg2+, respectively |
650012 |
| 3.1.4.35 | D714A |
33% and 22% of maximal activity in the absence of Mn2+ and Mg2+, respectively |
650012 |
| 3.1.4.35 | D754A |
59% and 28% of maximal activity in the absence of Mn2+ and Mg2+, respectively |
650012 |
| 3.1.4.35 | D762A |
mutant protein shows no cGMP hydrolytic activity |
667518 |
