EC Number   |
Protein Variants |
Reference |
|---|
   3.1.21.3 | D138A |
catalytically inactive |
694446 |
| 3.1.21.3 | D354A |
activity is significantly impaired |
691509 |
| 3.1.21.3 | E350A |
activity is significantly impaired |
-, 691509 |
| 3.1.21.3 | F353A |
activity is significantly impaired |
691509 |
| 3.1.21.3 | K220A |
the mutant enzyme shows a 5fold reduction in restriction activity. The mutant motor subunit is not defective in interacting with the methyltransferase to form the endonuclease complex |
751924 |
| 3.1.21.3 | K220Q |
the mutant enzyme shows a 400fold reduction in restriction activity. The mutant motor subunit is not defective in interacting with the methyltransferase to form the endonuclease complex |
751924 |
| 3.1.21.3 | K220R |
the mutant motor subunit is not defective in interacting with the methyltransferase to form the endonuclease complex |
751924 |
| 3.1.21.3 | L80P |
L80P mutation in the modification enzyme of the EcoRII gene complex confers thermosensitivity of cell growth (shows activity at 30°C but not at 37°C). Under a condition of inhibited protein synthesis, the activity of the mutant is completely lost at a high temperature. In parallel, the L80P mutant protein disappears more rapidly than the wild-type protein |
692848 |
| 3.1.21.3 | more |
a temperature-sensitive mutation within the hsdS gene appears to affect the ability of the HsdR subunit to interact with the HsdS subunit when forming an active endonuclease |
134246 |
| 3.1.21.3 | more |
gene of subunit hsdR from strain RN4220 contains a premature stop codon resulting in a truncated R.Sau1 product of 192 amino acids. Strain RN4220 can accept plasmid DNA from Escherichia coli. The truncated hsdR subunit is responsible for this phenotype and its complementation restores a nontransformable phenotype. Complemented strain RN4220 is resisitant to bacteriophage lysis if the phage is grown on staphylococcus aureus of a different lineage |
680463 |
