EC Number |
Protein Variants |
Reference |
---|
2.7.3.2 | A189D |
Km and kcat values similar to wild-type, T0.5 (°C): 46.2 (wild-type: 48.0°C) |
722399 |
2.7.3.2 | A205S |
Km and kcat values similar to wild-type, T0.5 (°C): 46.6 (wild-type: 48.0°C) |
722399 |
2.7.3.2 | A267H |
at pH 7.1 mutant shows 30% higher specific activity than the wild-type at 35°C, in contrast to mutant G268N this mutant shows no cold-adapted characteristics |
722077 |
2.7.3.2 | A267H |
Km and kcat values similar to wild-type, T0.5 (°C): 56.9 (wild-type: 56.9°C) |
722399 |
2.7.3.2 | A329S |
Km and kcat values similar to wild-type, T0.5 (°C): 56.6 (wild-type: 56.9°C) |
722399 |
2.7.3.2 | A76G |
mutation in the intra-subunit domain-domain interface, similar to wild-type in kinetics and thermal inactivation |
673658 |
2.7.3.2 | C146N |
Km and kcat values similar to wild-type, T0.5 (°C): 52.4 (wild-type: 56.9°C) |
722399 |
2.7.3.2 | C146S |
enzyme preparation contains only reduced form |
674788 |
2.7.3.2 | C254S |
enzyme preparation contains both oxidized and reduced forms |
674788 |
2.7.3.2 | C283S |
enzyme preparation contains both oxidized and reduced forms |
674788 |