EC Number |
Protein Variants |
Reference |
---|
2.3.3.16 | A10E |
site-directed mutagenesis, reduced kcat, increased Km for acetyl-CoA |
-, 488084 |
2.3.3.16 | A361R |
site-directed mutagenesis, slightly reduced kcat , reduced Km for acetyl-CoA and increased Km for oxaloacetate, enhanced activity with propionyl-CoA |
-, 488084 |
2.3.3.16 | A361R/A10E |
site-directed mutagenesis, reduced kcat, reduced Km for acetyl-CoA |
-, 488084 |
2.3.3.16 | D113A |
mutant with reduce thermostability |
719429 |
2.3.3.16 | D113A |
site-directed mutagenesis, deletion of C-terminal amino acid residues which arrange the subunit contact, slightly increased Km for acetyl-CoA and oxaloacetate, slightly increased reaction velocity, reduced thermostability |
488076 |
2.3.3.16 | D113S |
mutant with reduce thermostability |
719429 |
2.3.3.16 | D113S |
site-directed mutagenesis, deletion of C-terminal amino acid residues which arrange the subunit contact, slightly decreased Km for acetyl-CoA and oxaloacetate, slightly increased reaction velocity, reduced thermostability |
488076 |
2.3.3.16 | D12N |
reference for pI-value analysis |
702337 |
2.3.3.16 | D177A |
the mutant shows reduced activity compared to the wild type enzyme |
736463 |
2.3.3.16 | D317G |
D317 removes the acetyl-CoA methyl proton during catalysis |
702337 |