EC Number   |
Protein Variants |
Reference |
|---|
    1.1.1.42 | A134D |
the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme |
761477 |
| 1.1.1.42 | A325P/G326S |
50% inactivation after 10 min at 91.3°C as compared to 87.5°C for wild-type enzyme |
727545 |
| 1.1.1.42 | A336F |
50% inactivation after 10 min at 74°C as compared to 87.5°C or wild-type enzyme |
727545 |
| 1.1.1.42 | A741S |
the mutant exhibits higher specific activity and thermostability than the wild type enzyme |
761559 |
| 1.1.1.42 | C201M |
higher affinity for NAD+ than for NADP+ |
286804 |
| 1.1.1.42 | C201N |
higher affinity for NAD+ than for NADP+ |
286804 |
| 1.1.1.42 | C201V |
higher affinity for NAD+ than for NADP+ |
286804 |
| 1.1.1.42 | C245S |
the activities of the mutant in the presence of Cd2+ are decreased as that of wild type enzyme |
761531 |
| 1.1.1.42 | C269S |
activity of the C269S mutant is not affected by glutathione disulfide and no glutathionylated IDPc is observed with 5 mM glutathione disulfide confirming that Cys269 is a target of IDPc glutathionylation |
698059 |
| 1.1.1.42 | C269S |
site-directed mutagenesis, is not sensitive to inhibition by Cd2+ in contrast to the wild-type enzyme |
711158 |
