EC Number   |
Protein Variants |
Reference |
|---|
    1.1.1.284 | C100A |
mutant is misfolded |
-, 740859 |
| 1.1.1.284 | C11A |
about 55% of wild-type activity |
-, 740859 |
| 1.1.1.284 | C47S |
Cys47 is crucial to GFD activity |
740547 |
| 1.1.1.284 | C47S |
site-directed mutagenesis, active site mutant |
742700 |
| 1.1.1.284 | C48A |
about 5% of wild-type activity |
-, 740859 |
| 1.1.1.284 | D267E |
kinetic properties identical to that of wild-type enzyme |
287894 |
| 1.1.1.284 | D267E/T269I |
Km-value for S-hydroxymethylglutathione is about 11fold lower than that of the wild-type enzyme, turnover-number for S-hydroxymethylglutathione is about 8fold lower than that of wild-type enzyme |
287894 |
| 1.1.1.284 | D269I |
highly unstable enzyme, kinetic properties identical to that of wild-type enzyme |
287894 |
| 1.1.1.284 | D57L |
considerable loss of formaldehyde dehydrogenase activity |
287863 |
| 1.1.1.284 | D57L/Y93F |
fall in ratio kcat/Km for hydroxymethylglutathione by a factor of 1250, alcohol dehydrogenase activity of the mutant has gained a characteristic class I property, complete inhibition by 4-methylpyrazole at concentrations only partially reducing the activity of the wild-type class III enzyme |
287863 |
