EC Number   |
|---|
    6.1.1.15 | - |
| 6.1.1.15 | complexed with ATP and L-proline or halofuginone, hanging drop vapor diffusion method, using 0.1 M sodium cacodylate, 1.0 M sodium citrate dibasic trihydrate pH 6.5 |
| 6.1.1.15 | crystal structure analysis |
| 6.1.1.15 | enzyme complexed with 2 different tRNAPro isoacceptors, 2 weeks, 32% saturated ammonium sulfate, X-ray diffraction structure determination at 2.8-3.1 A resolution, superior quality crystal are obtained within 5-6 months by slowly precipitation and growth, crystal packing analysis |
| 6.1.1.15 | hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 6.5), 2 % (v/v) Tacsimate (pH 5.0), and 12% (w/v) polyethylene glycol 3350 |
| 6.1.1.15 | native enzyme or enzyme complexed with L-proline, tRNAPro isoacceptors, ATP-Mn2+, prolyl-adenylate intermediate, or prolyl-adenylate analogue Pro-AMS, preparation by soaking of native enzyme crystals in ligand solutions, X-ray diffraction structure determination at 2.85-3.0 A resolution |
| 6.1.1.15 | purified free enzyme or complexed with 3 tRNAPro isoacceptors, precipitant is 32% ammonium sulfate at pH 7.5, X-ray diffraction structure determination at 3.5 A resolution, and analysis |
| 6.1.1.15 | purified recombinant His-tagged wild-type and selenomethionine-labeled enzymes, hanging drop vapour diffusion method, mixing of protein solution containing 5 mg/ml with an equal volume of the reservoir solution containing 0.1 M citric acid, pH 5.5, 15%-17% PEG 3000, cryoprotection by 20% ethylene glycol, for PrsRp-adenylate analog complex cocrystals, the enzyme is mixed first with 500 nM ProAMS or 100 nM CysAMS, and then with an equal volume of a solution containing 0.1 M citric acid, pH 5.5, 10%-11% PEG 3000, 15%-20% ethylene glycol, X-ray diffraction structure determination and analysis at 2.9 A resolution |
| 6.1.1.15 | recombinant selenomethionine-labelled enzyme, expressed in Escherichia coli |
| 6.1.1.15 | sitting drop vapor diffusion method, using 10% (w/v) PEG-800, 20% (v/v) ethylene glycol, 0.1 M MES/imidazole, pH 6.5, and 0.03 M each sodium fluoride, sodium bromide, and sodium iodide |
