EC Number   |
|---|
   3.6.5.5 | GTPase domain and the bundle signalling element of dynamin in the GDP-bound state, GG1, hanging drop vapour diffusion method, mixing of 0.0015 ml of 10 mg/ml GG1 protein solution containing 2 mM GDP and 2 mM MgCl2 with 0.0015 ml of reservoir solution containing 0.1 M Tris, pH 8.0, 26% PEG 3350, and 0.2 M NaSCN, and equilibration against 0.7 ml of reservoir solution, one week, 4°C, X-ray diffraction structure determination and analysis at 1.7-1.8 A resolution, molecular replacement using GG1GDP.AlFx (PDB ID 2X2E) as search model |
| 3.6.5.5 | hanging-drop method, 1.9 A crystal structure of the nucleotide-free GTPase domain of dynamin 1 |
| 3.6.5.5 | purified recombinant dynamin-related protein 1 GTPase-GTPase effector domain fusion protein, sitting drop vapour diffusion method, mixing of 0.0004 ml of protein solution and reservoir solution, the latter containing 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 5.5, 25% v/v PEG 3350 or 0.1 M sodium citrate, pH 5.5, 20% PEG 3000, 20°C, X-ray diffraction structure determination and analysis at 2.67 A resolution |
| 3.6.5.5 | purified recombinant GST-tagged chimeric fusion protein of dynamin-related protein 1A, AtDRP1A, comprising GTPase domain of Arabidopsis thaliana AtDRP1A fused to its GTPase effector domain (GED) in a nucleotide-associated form, hanging drop vapour diffusion method, mixing of 5 mg/ml dimeric protein in 20 mM Tris-HCl, pH 8.0, 150 mM NaC, with 160 mM sodium formate, 16% w/v PEG 3350, 40 mM magnesium chloride, 6% w/v 2-propanol, 20 mM HEPES, pH 7.5, method optimization, X-ray diffraction structure determination and analysis at 3.6 A resolution, molecular replacement using the crystal structure of human dynamin GG dimer, PDB ID 2X2E |
| 3.6.5.5 | purified recombinant truncated enzyme Sey1p(1-692) , wild-type or selenomethionine-labeled, in complex with GDP, GMP-PNP, or GDP plus AlF4-, X-ray diffraction structure determination and analysis at 2.3-3.0 A resolution |
| 3.6.5.5 | purified wild-type and mutants of DNM1L isoform 2, mixing of 0.001 ml of 1.2 mg/ml protein with 0.001 ml of reservoir solution containing 0.1 M sodium citrate pH 5, 27.5% PEG 3000, equilibration against 0.4 ml of reservoir solution, 3-5 days, for co-crystallization of the enzyme with a non-hydrolyzable GTP analogue the protein is incubated with 1 mM GMP-PNP and 4 mM MgCl2, and then purified by gel filtration, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement using human dynamin-1 structure, PDB ID 3SNH |
