3.1.1.4 | four isozymes, for isozyme 1 the protein solution contains 10 mg/ml protein, 50 mM Tris-HCl, pH 8.5, and 3.5 M NaCl, for isozyme 2 7 mg/ml protein, 50 mM Tris-HCl, pH 8.5, and 2.8 M NaCl, for isozyme 3 5 mg/ml protein, 50 mM Tris-HCl, pH 8.5, and 2.8 M NaCl, and for isozyme 4 20 mg/ml protein, 50 mM TrisHCl, pH 8.5, and 2.4 M NaCl, X-ray diffraction structure determination and analysis at 2.5 A resolution, structure modeling |