EC Number   |
|---|
   2.8.2.2 | crystal structure analysis of both SULT2B1a and SULT2B1b in binary complexes with PAP (PDB IDs 1Q1Q, and 1Q1Z, respectively) have been resolved, as have two ternary complexes of SULT1Bb with PAP and pregnenolone (PDB ID 1Q2O), and with PAP and DHEA (PDB ID 1Q22) |
| 2.8.2.2 | DHEA-ST |
| 2.8.2.2 | homology modeling in complex with 3'-phosphoadenylyl sulfate |
| 2.8.2.2 | human cholesterol sulfotransferase SULT2B1b |
| 2.8.2.2 | purified recombinant mutants M137I and M137W, hanging drop vapor diffusion method at 18°C, 0.002 ml hanging drops by mixing equal volumes of protein solution and a reservoir solution containing 1.6 M (NH4)2SO4, 100 mM NaCl, and 0.1 M HEPES at pH 7.5, 3-7 days, X-ray diffraction structure determination and analysis at 2.6 and 3.0 A resolution, respectively, modeling |
| 2.8.2.2 | purified SULT1B1 is crystallized in the presence of 2 mM PAP using the hanging drop method at 20°C by mixing for SULT1B1: 0.002 ml of the protein solution with 0.002 ml of the reservoir solution containing 0.1 M Bis-Tris, pH 6.5, 0.2 M ammonium sulfate and 16%-20% PEG 4000 |
| 2.8.2.2 | several crystal structures of SULT2A1 binary complexes have been resolved, including complexes with DHEA (PDB ID 1J99) and PAPS (PDB ID 4IFB) |
