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EC Number Crystallization (Commentary)
Display the reaction diagram Show all sequences 2.1.2.B2enzyme complexed with various substrates including CMP, dCMP and 5'-hydroxymethyl-CMP, hanging drop vapor diffusion method, using 15% (w/v) polyethylene glycol 3350, 0.08 M lithium sulfate monohydrate and 0.1 M Tris-HCl, pH 8.5
Display the reaction diagram Show all sequences 2.1.2.B2structures of MilA and its complexes with substrates including CMP, dCMP and hydroxymethyl-CMP. Two residues in the active site of dCMP hydroxymethylase from T4 phage and thymidylate synthase from Escherichia coli, a serine and an arginine, are respectively replaced by an alanine and a lysine, Ala176 and Lys133, in MilA
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