EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
4.2.1.11 | 0.018 |
0.019 |
2-phospho-D-glycerate |
mutant enzyme S39A, pH 7.8, 21-23°C |
33603 |
4.2.1.11 | 15.47 |
- |
phosphoenolpyruvate |
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl |
701578 |
4.2.1.11 | 19.4 |
- |
2-phospho-D-glycerate |
isoform Err3p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication |
731032 |
4.2.1.11 | 19.7 |
- |
2-phospho-D-glycerate |
isoform Err2p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication |
731032 |
4.2.1.11 | 20.6 |
- |
2-phospho-D-glycerate |
recombinant, dimeric enzyme |
701914 |
4.2.1.11 | 22.2 |
- |
2-phospho-D-glycerate |
recombinant, His-tagged, hexameric enzyme |
701914 |
4.2.1.11 | 34.07 |
- |
phosphoenolpyruvate |
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl |
701578 |
4.2.1.11 | 37 |
- |
2-phospho-D-glycerate |
1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C |
730374 |
4.2.1.11 | 52.7 |
- |
2-phospho-D-glycerate |
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl |
701578 |
4.2.1.11 | 71.4 |
- |
2-phospho-D-glycerate |
isoform Eno1p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication |
731032 |