EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   3.2.2.27 | -999 |
- |
more |
excises 0.04 uracils per sec from uracil-mismatched oligonucleotide |
722532 |
| 3.2.2.27 | -999 |
- |
more |
kinetic parameters determined for Afung protein suggest a significantly lower level of enzymatic uracil release in Aeroglobus fulgidus as compared to the mesophilic Escherichia coli |
723240 |
| 3.2.2.27 | -999 |
- |
more |
rate constants of uracil excision determined for AFUDG and pseudo-FCL mutants using single-stranded uracil containing or double-stranded U:G base pair-containing substrates under single-turnover conditions |
721666 |
| 3.2.2.27 | 0.000072 |
- |
double stranded DNA containing G-U mismatch |
mutant enzyme H155S, at pH 7.6 and 60°C |
752224 |
| 3.2.2.27 | 0.0002 |
0.0003 |
fU-containing 10 nucleotide DNA sequence 5'-GGAGAfUCTCC-3' with opposing C, T, A, or G |
pH 7.5 |
708796 |
| 3.2.2.27 | 0.0014 |
- |
uracil-mismatched double-stranded DNA with U-G mismatch |
- |
691932 |
| 3.2.2.27 | 0.0075 |
- |
double stranded DNA containing G-U mismatch |
mutant enzyme E41Q/G42D, at pH 7.6 and 60°C |
752224 |
| 3.2.2.27 | 0.014 |
- |
uracil-mismatched double-stranded DNA with U-G mismatch |
pH 7.5, 37°C, wild-type enzyme |
694424 |
| 3.2.2.27 | 0.015 |
- |
5-hydroxymethyluracil-mismatched double-stranded DNA with U-A mismatch |
pH 7.5, 30°C, recombinant hSMUG1 |
692963 |
| 3.2.2.27 | 0.0175 |
- |
uracil-mismatched double-stranded DNA |
pH 7.5, 70°C |
723240 |
