EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    2.7.9.1 | -999 |
- |
- |
645434 |
| 2.7.9.1 | -999 |
- |
cold inactivation follows first order kinetics, this inactivation is protected by pyruvate, phosphoenolpyruvate and polyols, e.g. sucrose and glycerol |
645436 |
| 2.7.9.1 | -999 |
- |
cold labile, reactivation by several min incubation at 22ºC |
645442 |
| 2.7.9.1 | -999 |
- |
rapid inactivation at 0ºC |
645434 |
| 2.7.9.1 | -999 |
- |
up to 70% of activity of PPDK retained after incubation of transformed leaf extracts for 180 min on ice, 5fold increased amounts of recombinant enzyme by using a construct with seventeen amino acid substitutions, PPDK extracted from untransformed maize lost 90% of its activity after 20 min, in recombinant plants the photosynthetic rate (CO2 uptake) at 8°C significantly increased by 23%, no obvious effect at higher temperatures |
675905 |
| 2.7.9.1 | 0 |
- |
10 min 50% loss of activity |
645449 |
| 2.7.9.1 | 0 |
- |
20 min 82-87% loss of activity |
645449 |
| 2.7.9.1 | 0 |
- |
20 min, about 70% loss of activity |
645436 |
| 2.7.9.1 | 0 |
- |
45 min, 0.6 mg protein/ml, 78% loss of activity. 2.4 mg protein/ml, 29% loss of activity. Partial protection against cold inactivation with 10 mM MgCl2, 4 mM phosphoenolpyruvate, 1.52 mM glycerol, 0.48 mM proline, 0.23 mM betain, 0.40 mM sorbitol, 0.73 mM trehalose. Pyruvate has no effect |
645465 |
| 2.7.9.1 | 0 |
- |
5 min 50% loss of activity |
645449 |
