EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
2.7.1.107 | -999 |
- |
addition of phospholipids provides some protection from thermal inactivation |
640352 |
2.7.1.107 | 4 |
- |
after 6.5 h at 4°C, isoform DGKepsilon and DGKepsilonDELTA40 retain 19.1% and 15.8% of their initial activities, respectively, and 48.2% and 38.1% when stored in 50% glycerol, respectively |
760582 |
2.7.1.107 | 25 |
- |
recombinant purified isoform DGKepsilon and DGKepsilonDELTA40 are largely inactivated after being stored at room temperature for 7.5 h. After incubation at room temperature for 2 h, the proportions of activity that isoforms DGKepsilon and DGKepsilonDELTA40 retain are 35.2 and 50.3%, respectively |
760582 |
2.7.1.107 | 42 |
- |
5 min, complete loss of activity of DGK-II and DGK-III, about 10% loss of activity of enzyme form DGK-I |
640365 |
2.7.1.107 | 43 |
45 |
preincubation of microtubules, 10 min, 50% loss of activity |
640351 |
2.7.1.107 | 57 |
- |
Triton-solubilized preparation, t1/2: 12 min |
640352 |
2.7.1.107 | 100 |
- |
enzyme activity of membranes, t1/2: 20 min |
640352 |
2.7.1.107 | 100 |
- |
the membrane preparation shows a threefold stimulation by heating for 5 min followed by a gradual loss of activity, half-life: about 1 h. Butane-1-ol-dissolved enzyme has a half-life of about 45 min |
640360 |