EC Number   |
|---|
    5.4.99.7 | - |
| 5.4.99.7 | expressed in an ERG7-null mutant of Saccharomyces cerevisiae |
| 5.4.99.7 | expressed in Saccharomyces cerevisiae GIL77 cells |
| 5.4.99.7 | expressed in Saccharomyces cerevisiae strain erg7 |
| 5.4.99.7 | expression in Escherichia coli |
| 5.4.99.7 | expression in Pichia pastoris |
| 5.4.99.7 | expression in Saccharomyces cerevisiae |
| 5.4.99.7 | expression in yeast strains RXY6 and SMY8 |
| 5.4.99.7 | gene LAS1, recombinant expression in Solanum tuberosum leaves using the transformation method with Agrobacterium tumefaciens strain EHA 105. Overexpression of the Arabidopsis LAS in transgenic potato modifies phytosterol and steroidal glycoalkaloid metabolite levels and the transcript levels of the respective biosynthetic genes. LAS1 pverexpression in Solanum tnerosum leaves results in increased steroidal glycoalkaloid level and reduced phytosterol level in the leaves, while the steroidal glycoalkaloid level in the tuber flesh is reduced |
| 5.4.99.7 | identification of a putative LAS gene from potato (StLAS-like) and OSC activity verification in a yeast complementation assay by expression of the potato enzyme in LAS-deficient Saccharomyces cerevisiae strain GIL77. Analyses of StLAS-like transcript levels and StLAS-like::GUS reporter assays indicate different spatial expression in potato. Promoter sequence and activity analysis |
