EC Number   |
|---|
    4.2.1.118 | - |
| 4.2.1.118 | expression in Escherichia coli |
| 4.2.1.118 | gene asbF, sequence comparisons and phylogenetic analysis |
| 4.2.1.118 | gene qsuB, recombinant expression of the 3-dehydroshikimate dehydratase from Corynebacterium glutamicum in Arabidopsis thaliana transgenic C4H::qsuB lines, the enzyme is targeted to the plastids via plastid-targeting signal peptide (SCHL), reduces lignin content and improves biomass saccharification efficiency, reduction of lignin deposition. Compared to wild-type plants, lines expressing QsuB contain higher amount of protocatechuate, p-coumarate, p-coumaraldehyde and p-coumaryl alcohol, and lower amounts of coniferaldehyde, coniferyl alcohol, sinapaldehyde, and sinapyl alcohol. Metabolite analysis of C4H::qsuB lines, overview. Recombinant expression of YFP-tagged enzyme in plastids of transgenic Arabidopsis thaliana |
| 4.2.1.118 | gene quiC1, sequence comparisons and phylogenetic analysis |
| 4.2.1.118 | gene quiC1, sequence comparisons and phylogenetic analysis, plasmid-based expression of Pseudomonas putida QuiC1 in the Pseudomonas aeruginosa quiC1 knockout strain, recombinant expression of wild-type and mutant enzymes |
| 4.2.1.118 | in vivo overexpression of enzyme mutant Mut1 in Escherichiaxa0coli results in a about 60fold increase in functional enzyme when compared to the wild-type enzyme under the identical expression conditions, and 80-120% increase in DHB accumulation in the media |
| 4.2.1.118 | overexpression in Aspergillus nidulans |
