EC Number |
---|
3.6.5.5 | a 6-His N-terminal-tagged human dynamin2, lacking the C-terminal proline-rich domain, is constructed, cloned into the pET28a vector for expression in Escherichia coli BL21DE3 cells |
3.6.5.5 | a CHO-K1 cDNA library in the pSPORT vector is screened, the PCR product, derived from a positive clone, is cloned using the vectors pcDNA3.1Zeo+Myc6-, pcDNA3.1Zeo+Flag-, pcDNA3.1Zeo+HA2-, and pGEX6P-2, DLP1 cDNA from ZP121 is subcloned into pGEM-Teasy |
3.6.5.5 | adenovirus expressing the dynamin mutant K44A is constructed |
3.6.5.5 | DNA encoding the full-length Dnm1 protein is cloned into the pMALc2x vector for expression in Escherichia coli Rosetta cells |
3.6.5.5 | Dyn1 expression in bovine adrenal chromaffin cells, coexpression with granule marker VMAT2-pH, the amount of dynamin on the plasma membrane in dynamin-transfected cells was increased 2.5 to 3.5 times compared with endogenous dynamin in nontransfected cells |
3.6.5.5 | dynamin 2 genotyping |
3.6.5.5 | expressed in a fibroblastoid cell line and in embryonic stem cells |
3.6.5.5 | expressed in CHO cells |
3.6.5.5 | expressed in HEK-293 cells |
3.6.5.5 | expressed in HeLa cells |