EC Number |
---|
3.4.24.B2 | from a Bacillus amyloliquefaciens 23-7A library |
3.4.24.B2 | gene GT-SM3B, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of the soluble enzyme with introduced N-terminal hexahistidine thrombin-cleavable tag in Escherichia coli strain BL21 (DE3) cytoplasm and secretion of the enzyme |
3.4.24.B2 | gene pepF, DNA and amino acid sequence determination and analysis |
3.4.24.B2 | gene pepF, DNA sequence determination and analysis, 2 putative promotor regions, operon expression is independent of the transcription regulator CtrA |
3.4.24.B2 | overexpression in Bacillus subtilis |
3.4.24.B2 | screening of a EZ-Tn5 transposon random mutant library and cloning of gene pepF, DNA and amino acid sequence determination and analysis, real-time quantitative PCR expression analysis |