EC Number   |
|---|
   3.1.1.76 | a stationary sigma factor (rpoS)negative mutant is constructed to evaluate polyhydroxyalkanoate accumulation and expression of a translational fusion to the promoter region of the genes that code for polyhydroxyalkanoate synthase 1 (phaC1) and polyhydroxyalkanoate depolymerase (phaZ). The rpoS gene of Pseudomaonas putida KT2440 and its promoter region are amplified with primers and cloned into pBBR1MCS-5 to obtain pBBRrpoS. This plasmid is transferred to Pseudomonas putida SDM75 and SDMPC1. |
| 3.1.1.76 | expression in Escherichia coli |
| 3.1.1.76 | expression in Rhodococcus sp. |
| 3.1.1.76 | gene phaZ, DNA and amino acid sequence determination and analysis, expression and subcloning in Escherichia coli strain DH5alpha |
| 3.1.1.76 | gene phaZ, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) in inclusion bodies |
| 3.1.1.76 | gene phaZ, functional expression in Escherichia coli strain JM109 |
| 3.1.1.76 | gene phaZSomSO2, DNA and amino acid sequence determination and analysis |
| 3.1.1.76 | gene phaZSroSL3, DNA and amino acid sequence determination and analysis |
| 3.1.1.76 | gene phaZSveSO1, DNA and amino acid sequence determination and analysis |
| 3.1.1.76 | in Escherichia coli |
