EC Number |
---|
3.1.1.4 | - |
3.1.1.4 | basic-acidic hybrid PLA2 cDNA, cloned into the expression vector pBLMVL2 for later transformation in Escherichia coli |
3.1.1.4 | C-terminal His6-tagged GIVA PLA2 expressed using recombinant baculovirus in a suspension culture of Sf9 insect cells |
3.1.1.4 | cDNA cloned into the pGEM-T vector and transformed into Escherichia coli strain DH5alpha |
3.1.1.4 | cDNA coding for the predicted mature PLA2 protein, amino acids 1-128 without the signal peptide, designed with optimized codons for high level expression in Escherichia coli strain Tuner (DE3) LacI |
3.1.1.4 | CHO-K1 cells or HEK cells stably expressing N-terminal enhanced green fluorescent protein-cPLA2alpha fusion protein |
3.1.1.4 | chromosomal location at 1G1, phylogenetic tree |
3.1.1.4 | cPLA2-alpha and sPLA2s |
3.1.1.4 | cPLA2gamma, expressed in Sf9 cells, lung fibroblasts and KEK293 cells |
3.1.1.4 | DNA and amino acid sequence determination and analysis |