EC Number   |
|---|
    2.7.9.1 | - |
| 2.7.9.1 | 25 kDa C-terminal and 35 kDa N-terminal deletion mutants expressed in Escherichia coli |
| 2.7.9.1 | 78% of homology with maize enzyme |
| 2.7.9.1 | codon-optimized coding regions of PPDK from Flaveria trinervia stripped of the chloroplast transport sequence are cloned into the multiple cloning site of a pET-16b vector (Novagen) containing a His10 tag and coding for a Tobacco Etch Virus protease cleavage site. Escherichia coli BL21 (DE3) cells are transformed with this plasmid |
| 2.7.9.1 | Escherichia coli |
| 2.7.9.1 | Escherichia coli, strains DH5alpha and BL21-CodonPLUS(DE3)-RIL |
| 2.7.9.1 | Escherichia coli, three constructs, one construct consisting of the 3'-part of Flaveria brownii (Asteraceae) of cold tolerant PPDK fused to maize PPDK (15th exon), another construct includes a set of point mutations to substitute all of the 17 residues that differ between the 3'-parts of maize and Flaveria brownii PPDK, respectively, the whole genomic sequence of the maize PPDK gene is included as a control, transformation of constructs into maize inbred line A188 by Agrobacterium tumefaciens (strain LBA4404), individual range of variation in the amount of PPDK among regenerated plants, crude leaf extracts of some transformed plants produce a large amount of cold tolerant recombinant enzyme and reveal a greatly improved cold tolerance especially by using the construct altered at 17 amino acid positions |
| 2.7.9.1 | expressed in a baculovirus system |
| 2.7.9.1 | expressed in Arabidopsis thaliana, found exclusively in chloroplasts of transgenic Arabidopsis plants |
| 2.7.9.1 | expressed in Escherichia coli |
