EC Number   |
|---|
   2.7.7.65 | 630-bp internal fragment from bacterial DNA in suicide plasmid pRL277 for construction of suicide plasmid pAM4108 and generation of mutant strains by homologous recombination, complete ORF in pAM2770 for generation of pAM4114 for complementation studies, complete ORF in pEZ-30b(+) (pAM4097) for expression with C-terminal hexa-His-tag in Escherichia coli BL21(DE3) |
| 2.7.7.65 | a DNA construct encoding the C-terminus of the TM1788 gene is cloned into the vector pET28b+ for expression in Escherichia col BL21DE3 cells |
| 2.7.7.65 | addition of a C-terminal His-tag to GcbC results in a loss of function and stability |
| 2.7.7.65 | cloning and expression of gene LIC13137 (Lcd1), gene fragment LIC13137GAF1-175 (Lcd1GAF), and gene fragment LIC13137GAF1-180 as His-tagged proteins in Escherichia coli strain BL21(DE3)RP |
| 2.7.7.65 | expressed as a His-tagged fusion protein in Escherichia coli |
| 2.7.7.65 | expressed in Escherichia coli as a His-tagged fusion protein |
| 2.7.7.65 | expression as GFP fusion protein for subcellular targeting, expression with His-tag in Escherichia coli |
| 2.7.7.65 | expression in a cyanobacterial system |
| 2.7.7.65 | expression in Escherichia coli with His-tag |
| 2.7.7.65 | expression in Pseudomonas aeruginosa PAO1 |
