EC Number   |
|---|
    1.13.11.34 | - |
| 1.13.11.34 | 5-lipoxygenase cDNA cloned into EGFP fusion protein vector (pEGFP-h5-LO) and expressed in HEK293/T or COS-7 cells, or into a pUC13 vector, full length 5-lipoxygenase cDNA subcloned into pGEX and pcDNA3, expressed in Escherichia coli Y1090 |
| 1.13.11.34 | detection of alternatively spliced 5-LO transcripts in B and T cell lines and in primary cells encoding naturally occuring mutant 5-LO catalytically inactive isoforms 5-LODELTA13, 5-LODELTA4 and 5-LOp12, that lack the exons 13, 4 or a part of exon 12, respectively, RT-PCR expression analysis of 5-LO-WT and isoform expression. Coexpression of the truncated natural isoforms with 5-LO-wild-type in transiently and stably transfected HEK-293T cells |
| 1.13.11.34 | DNA and amino acid sequence analysis |
| 1.13.11.34 | Escherichia coli MV1190 transformed with pT3-5LO plasmid |
| 1.13.11.34 | expressed in Escherichia coli |
| 1.13.11.34 | expressed in Escherichia coli as a His-tagged fusion protein |
| 1.13.11.34 | expressed in Escherichia coli BL21 and MV1190 cells |
| 1.13.11.34 | expressed in Sf-21 insect cells |
| 1.13.11.34 | expression in Escherichia coli |
