EC Number |
Storage Stability |
---|
2.7.1.30 | -20°C, 50% glycerol, stable for more than 1 year |
2.7.1.30 | -20°C, pH 5.0, partially purified enzymes have half-lives of several weeks to months |
2.7.1.30 | 0°C, suspension of crystals, 10 mM glycerol, 1 mM EDTA, 1 mM 2-mercaptoethanol, 0.1 M potassium phosphate, pH 7.0, saturated with ammonium sulfate, stable for several years |
2.7.1.30 | 4°C, crystallized suspension in 2.2 M ammonium sulfate, stable for several months |
2.7.1.30 | 4°C, in 0.1 M sodium phosphate buffer pH 6.8 containing 10 mM MgSO4, 48 h, more than 50% loss of activity |
2.7.1.30 | 4°C, in 0.1 M sodium phosphate buffer pH 6.8 containing 10 mM MgSO4, 5 mM ADP, 10 mM MgSO4 and 0.1% (v/v) glycerol, 4 weeks, no loss of activity |
2.7.1.30 | as crystalline suspension in saturated ammonium sulfate, solutions containing 10 mM glycerol, 1 mM EDTA and a thiol e.g. 2-mercaptoethanol, yeast enzyme stable for several months, E. coli enzyme for several years |