EC Number   |
Storage Stability |
|---|
    1.1.1.1 | -20°C, 1 M NaCl in 20 mM Tris-HCl (pH 8.4) in the presence of an equal volume of glycerol, 12 months, no loss of activity |
| 1.1.1.1 | -20°C, 10 mM potassium phosphate buffer, pH 7.0, containing 0.5 mM NAD+ without loss of activity for several months |
| 1.1.1.1 | -20°C, in the presence of 50% (v/v) glycerol and at highly concentrated solutions (0.75-1 mg protein/ml), 3 days, 40-50% loss of activity |
| 1.1.1.1 | -20°C, inactive after approximately 2 weeks, storage at -80°C or lyophilization does not improve storage time |
| 1.1.1.1 | -20°C, isoenzyme 2 is stable in 50% ethylene glycol for at least 3 months, isoenzyme 1 and 3 maintain less than 50% of their original activity after 2 weeks |
| 1.1.1.1 | -20°C, several months, no loss of activity |
| 1.1.1.1 | 15°C, 50 mM sodium phosphate buffer, pH 8.0, half-life of wild-type 7 h, half-life of mutant C257L 17 h |
| 1.1.1.1 | 4°C, 1 M NaCl in 20 mM Tris-HCl (pH 8.4) in the absence of glycerol, 4 months, 60% loss of activity |
| 1.1.1.1 | 4°C, 10 mM HEPES buffer, 1 mM dithioerythritol, pH 7.5, stable for 2 weeks |
| 1.1.1.1 | 4°C, 5 mM Na phosphate, pH 7.5, the half-life is 24 h. 0.01 mM ethanol effectively stabilizes for several weeks |
