6.1.1.3 | recombinant mutant M129K D-aminoacyl-tRNA deacylase-like domain from inclusion bodies after expression in Escherichia coli strain BL21(DE3) in 0.1 M phosphate buffer at pH 8.0 containing 6 M guanidinium hydrochloride and 10 mM Tris-HCl, followed by nickel affinity chromatography, the denatured protein is refolded stepwise by dialysis in 0.1 M phosphate buffer, pH 8.0, containing 10 mM Tris-HCl |
673326 |