EC Number |
---|
4.1.99.5 | - |
4.1.99.5 | 208fold, solubilized with octyl beta-glucoside |
4.1.99.5 | partial, 1306fold, solubilized with beta-octyl glucoside |
4.1.99.5 | recombinant enzyme from Escherichia coli |
4.1.99.5 | recombinant enzyme mutants V41Y and A134F to homogeneity |
4.1.99.5 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) in an anaerobic chamber, further by metal affinity chromatography |
4.1.99.5 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration, followed by ultrafiltration |
4.1.99.5 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and dialysis |
4.1.99.5 | recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography and dialysis |
4.1.99.5 | recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, removal of the tag by thrombin, and gel filtration |