EC Number   |
|---|
   3.4.24.B17 | - |
| 3.4.24.B17 | coiled-coil C-terminus, residues 541-585, as His-tagged peptide |
| 3.4.24.B17 | HiTrapQ column chromatography and Superdex 200 gel filtration |
| 3.4.24.B17 | Ni-NTA agarose column chromatography, and gel filtration |
| 3.4.24.B17 | recombinant enzyme, solubilization by N-lauylsarcosine, refolding in presence of ATP, Mg2+ and Zn2+, and purification to near homogeneity |
| 3.4.24.B17 | recombinant Hi-Myc-tagged enzyme |
| 3.4.24.B17 | recombinant His-Myc-tagged wild-type and mutant enzymes |
| 3.4.24.B17 | recombinant His-Myc-tagged wild-type and mutants |
| 3.4.24.B17 | recombinant His-tagged enzyme from Escherichia coli |
| 3.4.24.B17 | recombinant soluble fusion proteins, more than 80% homogeneity |
