EC Number   |
|---|
   3.4.22.56 | - |
| 3.4.22.56 | Mono-Q column chromatography and Superose 12 gel filtration |
| 3.4.22.56 | Ni-NTA agarose column chromatography and Resource Q column chromatography |
| 3.4.22.56 | recombinant enzyme from Escherichia coli by nickel affinity chromatography, ion exchange chromatography, and gel filtration |
| 3.4.22.56 | recombinant enzyme, inclusion bodies produced in Escherichia coli |
| 3.4.22.56 | recombinant His6-tagged caspase-3 from Escherichia coli by nickel affinity chromatography |
| 3.4.22.56 | upon purification and activation, caspase-3 subunits A and B are processed to yield the sequences S29GIS...IETD175 and S176GVD
YFYH277-(H)7, the purified enzyme shows 90% of maximum activity |
