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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the reaction diagram Show all sequences 3.1.1.56-
Display the reaction diagram Show all sequences 3.1.1.56by nickel-affinity chromatography (elution: 300 mM imidazole) followed by cleavage of His-tag by tobacco etch virus (TEV) protease, a second nickel-affinity chromatography, and size-exclusion chromatography (Superdex G75) in presence of 1 mM dithiothreitol
Display the reaction diagram Show all sequences 3.1.1.56isoenzyme Es D1 and Es D2
Display the reaction diagram Show all sequences 3.1.1.56partial, esterase D is identical to sialic acid-specific O-acetylesterase
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