EC Number   |
|---|
   2.3.1.82 | - |
| 2.3.1.82 | both acetyltransferase domains of the bifunctional enzyme AAC(3)-Ib/AAC(6')-Ib' are cloned and purified |
| 2.3.1.82 | His-tagged AAC(6')-Isa |
| 2.3.1.82 | Ni-NTA column chromatography and Superdex S200 gel filtration |
| 2.3.1.82 | on a His-Bind NiNTA column |
| 2.3.1.82 | on a Ni-NTA and a Superdex S-75 column, the His6 tag is cleaved using thrombin |
| 2.3.1.82 | partial |
| 2.3.1.82 | recombinant AAC(6')-Iaf is purified using Ni-nitrilotriacetic acid agarose, native AAC(6')-Iaf from Pseudomonas aeruginosa on an anti-AAC(6')-Iaf-IgG coupled and NHS-activated Sepharose column |
| 2.3.1.82 | strain GN315 |
| 2.3.1.82 | unseparable from 2-O-aminoglycoside phosphotransferase by affinity chromatography or sucrose density gradient ultracentrifugation |
