EC Number   |
|---|
    1.8.1.4 | - |
| 1.8.1.4 | affinity chromatography on propyllipoamide-glass columns |
| 1.8.1.4 | ammonium sulfate precipitation |
| 1.8.1.4 | ammonium sulfate precipitation and Ni-NTA agarose column chromatography |
| 1.8.1.4 | by gel filtration |
| 1.8.1.4 | DEAE-Toyopearl 650 M column chromatography, butyl-Toyopearl 650 M column chromatography, and TSK-gel G3000SW gel filtration |
| 1.8.1.4 | dissociation of the lipoamide dehydrogenase component from the branched-chain alpha-keto acid dehydrogenase complex during purification |
| 1.8.1.4 | enzmye from culture supernatant of Clostridium kluyveri is purified using a DEAE-toyopearl column and a TSK-GEL G-3000 column. Recombinant protein is purified using a HiTrap chelating HP column |
| 1.8.1.4 | from pyruvate dehydrogenase complex |
| 1.8.1.4 | further purification of the commercial preparation |
