EC Number |
---|
1.4.4.2 | - |
1.4.4.2 | H1, H2, and L protein purified to homogeneity, more than 95% pure |
1.4.4.2 | Ni-NTA column chromatography |
1.4.4.2 | Q-Sepharose column chromatography and Sephacryl S-100 gel filtration |
1.4.4.2 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity and ion exchange chromatography, and gel filtration |
1.4.4.2 | recombinant His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis |
1.4.4.2 | ultracentrifugation |
1.4.4.2 | using a nickel-nitrilotriacetic acid-agarose column |