EC Number   |
|---|
   1.3.7.5 | - |
| 1.3.7.5 | by gel filtration |
| 1.3.7.5 | recombinant enzyme from Escherichia coli strain BL21(DE3) by anion-exchange chromatography, hydrophobic interaction chromatography, hydroxyapatite chromatography, and gel filtration |
| 1.3.7.5 | recombinant GST-tagged PcyA from Escherichia coli by glutathione affinity chromatography |
| 1.3.7.5 | recombinant His-tagged enzyme from Escherichia coli strain C41 by nickel affinity chromatography and dialysis |
| 1.3.7.5 | recombinant mutant enzymes from Escherichia coli strain BL21(DE3) by cyanide affinity chromatography |
| 1.3.7.5 | recombinant PcyA |
| 1.3.7.5 | recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) |
| 1.3.7.5 | Superdex G-200 gel filtration |
| 1.3.7.5 | wild-type and mutants, histidine mutants purified to homogeneity |
