3.4.24.B26 | synthesis |
optimization of the culture conditions for protease production, best conditions are (w/v) 1% corn powder, 4% wheat bran, 2% bean powder, 0.4% Na2HPO4, 0.03% KH2PO4, 0.1% CaCl2, and artificial seawater, with the initial pH of 8.0 and 1% inoculation amount, 15°C culture temperature. Under the optimized conditions, the protease activity reaches 1137 U/ml, i.e., 174% of that before optimization. The protease activity in small scale fermentations reaches 1546.4 U/ml after parameter optimization |
-, 754793 |