EC Number   |
Application |
Reference |
|---|
  3.2.1.B34 | biotechnology |
construction of a series of Sulfolobus-Escherichia coli shuttle vectors based on the small multicopy plasmid pRN1 from Sulfolobus islandicus. The shuttle vectors do not integrate into the genome and do not rearrange. They allow functional overexpression of genes, and the beta-glycosidase (lacS) gene of ulfolobus solfataricus could function as selectable marker in Suldfolobus solfataricus |
-, 736898 |
| 3.2.1.B34 | synthesis |
enzyme is a potential producer of the rare ginsenosides compound K, compound Y, and compound Mc from the major ginsenosides Rb1, Rb2, Rc, and Rd |
-, 729348 |
| 3.2.1.B34 | synthesis |
use of alpha-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus, EC 3.2.1.55, along with beta-glycosidase from Sulfolobus solfataricus to produce ginsenoside compound K from the protopanaxadiol-type ginsenosides in red-ginseng extract. The optimal reaction conditions are as follows: pH 6.0, 80°C, 2 U/ml Sulfolobus solfataricus enzyme, 3 U/ml Caldicellulosiruptor saccharolyticus enzyme, and 7.5 g/l protopanaxadiol-type ginsenosides. The enzymes produce 4.2 g/l ginsenoside compound K from 7.5 g/l ginsenosides in 12 h without other ginsenosides, with a molar yield of 100% and a productivity of 348 mg/l/h |
-, 735035 |
