EC Number |
Natural Substrates |
---|
1.17.7.4 | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ |
- |
1.17.7.4 | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ |
formation of a mixture of isopentenyl diphosphate and dimethylallyl diphosphate in a ratio 1:5 |
1.17.7.4 | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ |
HMBPP reductase catalyzes the 2H+/2e- reduction of (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate to form an approximately 5:1 mixture of isopentenyl diphosphate and dimethylallyl diphosphate, insights into IspH catalysis and inhibition, involving organometallic species. Residue E126 is essential for catalytic activity. Residue H124 is not a major contributor to substrate binding, but is essential for catalysis, it is involved in delivering H+ to E126 and the bound (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate. Residue H42 forms hydrogen bonds to the bound diphosphate ligand. Ligand binding structure spectral analysis and modelling, overview |
1.17.7.4 | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ |
in the nonmevalonate pathway, the terminal intermediate, 1-hydroxy-2-methyl-2-butenyl 4-diphosphate, i.e. HMBPP, is reductively converted into a mixture of isopentenyl diphosphate and dimethylallyl diphosphate |
1.17.7.4 | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + reduced ferredoxin [iron-sulfur] cluster + H+ |
- |
1.17.7.4 | isopentenyl diphosphate + NAD(P)+ + H2O |
part of isoprenoid biosynthesis |
1.17.7.4 | isopentenyl diphosphate + NAD(P)+ + H2O |
biosynthesis of terpenes |
1.17.7.4 | isopentenyl diphosphate + NAD(P)+ + H2O |
2-C-methyl-D-erithritol-4-phosphate pathway |
1.17.7.4 | isopentenyl diphosphate + NAD(P)+ + H2O |
investigation of biosynthetic coupling and pattern by expression of involved genes from synthetic operon |
1.17.7.4 | more |
production of isopentenyl diphosphate and dimethylallyl diphosphate is key step in controlling the production of plastidial isoprenoid precursors and carotenoid biosynthesis in plant cells |