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EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Reference
3.1.11.1
-999
-
more
kinetics of the enzyme located in streptavidin-coated microspheres with biotinylated DNA substrate and Mg2+ cofactor
664336
3.1.11.1
0.016
-
single-stranded DNA
at 37°C, in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 3 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 10% (v/v) glycerol, 5% (v/v) dimethyl sulfoxide
707503
3.1.11.1
0.04
-
oligonucleotide (polydT)(polydA)(polydT)(polydA)
-
133945
3.1.11.1
0.1
-
oligonucleotide (polydA)4
inverse dependence with polymer size
133944
3.1.11.1
0.13
-
ssDNA 50 mer
pH 7.5, 37°C
710152
3.1.11.1
0.16
-
ssDNA 50 mer
pH 7.5, 60°C
710152
3.1.11.1
0.24
-
ssDNA 40 mer
pH 7.5, 37°C
710152
3.1.11.1
0.26
-
ssDNA 28 mer
pH 7.5, 37°C
710152
3.1.11.1
0.28
-
ssDNA 10 mer
pH 7.5, 37°C
710152
3.1.11.1
0.29
-
ssDNA 28 mer
pH 7.5, 60°C
710152
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