EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    1.13.11.34 | -999 |
- |
more |
kinetics with lysophospholipid substrates |
685330 |
| 1.13.11.34 | -999 |
- |
more |
Michaelis-Menten steady-state kinetics, kinetic isotope effect of 5-LOX, detailed overview. The reaction of 5-LOX with unlabeled arachidonate generates products from hydrogen abstraction at C7 (95% 5-HETE) with minor products derived from abstraction at C10 (5% 8-HETE). With 7,7-d2-arachidonate the selectivity remains skewed towards C7 hydrogen abstraction (59%) compared to C10 hydrogen abstraction products (41% 8-HETE), but the selectivity is significantly reduced compared to unlabeled arachidonate. 5-LOX exhibits isotope sensitive branching, shift in regiospecificity induced by deuterium-labeling. Dependence of the steady-state velocity on arachidonate concentration follows Michaelis-Menten kinetics up to 0.04-0.05 mM, effects of temperature and viscosity |
742078 |
| 1.13.11.34 | -999 |
- |
more |
steady-state kinetics and kinetic mechanism, overview. Kinetic parameters for ATP-induced activation of 5-LOX |
741898 |
| 1.13.11.34 | 0.002 |
- |
1-linoleoyl lysophosphatidic acid |
pH 6.3, 25°C |
685330 |
| 1.13.11.34 | 0.003 |
- |
1-linoleoyl lysophosphatidylcholine |
pH 6.3, 25°C |
685330 |
| 1.13.11.34 | 0.0033 |
- |
arachidonate |
pH 8.0, temperature not specified in the publication |
764178 |
| 1.13.11.34 | 0.004 |
- |
linoleic acid |
pH 6.3, 25°C |
685330 |
| 1.13.11.34 | 0.0051 |
- |
arachidonate |
reaction mixture contains 1 mM DTT, 1 mM ATP, 2 mM CaCl2, and 0.01 mM indomethacin in phosphate buffered saline, at 37°C |
673996 |
| 1.13.11.34 | 0.0093 |
- |
7,7-d2-arachidonate |
pH 7.5, 25°C, HPLC analysis, 8-HETE formation |
742078 |
| 1.13.11.34 | 0.01144 |
- |
7,7-d2-arachidonate |
pH 7.5, 25°C, UV-Vis spectrophotometric analysis |
742078 |
