Information on EC 6.3.2.28 -

Information on EC 6.3.2.28 - L-amino-acid alpha-ligase:

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EC NUMBER	COMMENTARY
6.3.2.28	-

RECOMMENDED NAME	GeneOntology No.
L-amino-acid alpha-ligase	GO:0034026

REACTION	REACTION DIAGRAM	COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
ATP + an L-amino acid + an L-amino acid = ADP + phosphate + L-aminoacyl-L-amino acid		-	-	-	-
ATP + an L-amino acid + an L-amino acid = ADP + phosphate + L-aminoacyl-L-amino acid		-	Bacillus subtilis	-	662037

REACTION TYPE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

PATHWAY	KEGG Link	MetaCyc Link
No entries in this field

SYSTEMATIC NAME

IUBMB Comments

L-amino acid:L-amino acid ligase (ADP-forming)

The enzyme from Bacillus sp. requires Mg2+ or Mn2+ for activity. While the enzyme has extremely broad substrate specificity, it does not accept highly charged amino acids, such as Lys, Arg, Glu and Asp, nor does it react with secondary amines such as Pro. The N-terminal residue of the alpha-dipeptide formed seems to be limited to Ala, Gly, Ser, Thr and Met (with Ala and Ser being the most preferred), whereas the C-terminal residue seems to allow for a wider variety of amino acids (but with a preference for Met and Phe). However, not all combinations or dipeptides are formed. For example, while Ser is acceptable for the N-terminus and Thr for the C-terminus, a Ser-Thr dipeptide is not formed. D-Ala, D-Ser and D-Phe are not substrates. Belongs in the ATP-dependent carboxylate-amine/thiol ligase superfamily.

SYNONYMS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
BAD_1200	Bifidobacterium adolescentis	A1A2P8	gene name	714524
BL00235	Bacillus licheniformis	-	-	693286
BL02410	Bacillus licheniformis	Q65P25	gene name	714524
CV_0806	Chromobacterium violaceum	-	gene name	714524
Haur_2023	Herpetosiphon aurantiacus	A9AVH5	gene name	714524
L-amino acid alpha-ligase	Actinobacillus pleuropneumoniae	-	-	702771
L-amino acid alpha-ligase	Photorhabdus luminescens subsp. laumondii	Q7N7D2	-	702771
L-amino acid alpha-ligase	Streptococcus mutans	-	-	702771
L-amino acid alpha-ligase	Streptococcus pneumoniae	Q97RD7	-	702771
L-amino acid alpha-ligase	Treponema denticola	Q73KK9	-	702771
L-amino acid ligase	Bacillus licheniformis	-	-	693286
L-amino acid ligase	Bacillus subtilis	-	-	702767, 702768
LAL	Ralstonia solanacearum	B2DEW2	-	690733
LAL	Actinobacillus pleuropneumoniae	-	-	702771
LAL	Photorhabdus luminescens subsp. laumondii	Q7N7D2	-	702771
LAL	Streptococcus mutans	-	-	702771
LAL	Streptococcus pneumoniae	Q97RD7	-	702771
LAL	Treponema denticola	Q73KK9	-	702771
RizA	Bacillus subtilis	B5UAT8	-	702767
rizB	Bacillus subtilis	-	gene name	714524
RSp1486a	Ralstonia solanacearum	B2DEW2	-	690733
spr0969	Streptococcus pneumoniae	-	gene name	714524
YwfE	Bacillus subtilis	-	-	662037

CAS REGISTRY NUMBER	COMMENTARY
No entries in this field

ORGANISM	COMMENTARY	LITERATURE	SEQUENCE CODE	SEQUENCE DB	SOURCE
Actinobacillus pleuropneumoniae	-	702771	-	-
Bacillus licheniformis	putative	714524	Q65P25	UniProt
Bacillus licheniformis	strain NBRC12200, gene BL00235	693286	-	-
Bacillus licheniformis NBRC 12200	putative	714524	Q65P25	UniProt
Bacillus licheniformis NBRC12200	strain NBRC12200, gene BL00235	693286	-	-
Bacillus subtilis	-	662037, 671498, 714524	-	-
Bacillus subtilis	NBRC3134	702767	B5UAT8	UniProt
Bacillus subtilis	NBRC3134	702768	C0STU2	UniProt
Bacillus subtilis NBRC 3134	-	714524	-	-
Bacillus subtilis NBRC3134	NBRC3134	702767	B5UAT8	UniProt
Bacillus subtilis NBRC3134	NBRC3134	702768	C0STU2	UniProt
Bifidobacterium adolescentis	-	714524	A1A2P8	UniProt
Bifidobacterium adolescentis JCM 1275	-	714524	A1A2P8	UniProt
Chromobacterium violaceum	-	714524	-	-
Chromobacterium violaceum NBRC 12614	-	714524	-	-
Herpetosiphon aurantiacus	putative	714524	A9AVH5	UniProt
Herpetosiphon aurantiacus ATCC 23779	putative	714524	A9AVH5	UniProt
Photorhabdus luminescens subsp. laumondii	-	702771	Q7N7D2	UniProt
Ralstonia solanacearum	strain JCM 10489, gene lal	690733	B2DEW2	UniProt
Ralstonia solanacearum JCM 10489	strain JCM 10489, gene lal	690733	B2DEW2	UniProt
Streptococcus mutans	-	702771	-	-
Streptococcus pneumoniae	-	702771	Q97RD7	UniProt
Streptococcus pneumoniae	-	714524	-	-
Streptococcus pneumoniae ATCC BAA-255	-	714524	-	-
Treponema denticola	-	702771	Q73KK9	UniProt

GENERAL INFORMATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
physiological function	Bacillus subtilis	C0STU2	involved in rhizocticin biosynthesis	702768

SUBSTRATE	PRODUCT	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY/ Substrate	LITERATURE/ Substrate	COMMENTARY/ Product	LITERATURE/ Product	Reversibility r=reversible ir=irreversible ?=not specified
2 ATP + 3 L-Ile	2 ADP + 2 phosphate + L-isoleucyl-L-isoleucyl-L-isoleucine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Ile	-	?
2 ATP + 3 L-Ile	2 ADP + 2 phosphate + L-isoleucyl-L-isoleucyl-L-isoleucine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetramers of L-Ile	-	?
2 ATP + 3 L-Ile	2 ADP + 2 phosphate + L-isoleucyl-L-isoleucyl-L-isoleucine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetramers of L-Ile	-	?
2 ATP + 3 L-Ile	2 ADP + 2 phosphate + L-isoleucyl-L-isoleucyl-L-isoleucine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetramers of L-Ile	-	?
2 ATP + 3 L-Ile	2 ADP + 2 phosphate + L-isoleucyl-L-isoleucyl-L-isoleucine		Herpetosiphon aurantiacus	A9AVH5	-	714524	products are di-, trimers of L-Ile	-	?
2 ATP + 3 L-Ile	2 ADP + 2 phosphate + L-isoleucyl-L-isoleucyl-L-isoleucine		Chromobacterium violaceum	-	-	714524	products are dimers of L-Ile	-	?
2 ATP + 3 L-Leu	2 ADP + 2 phosphate + L-leucyl-L-leucyl-L-leucine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Leu	-	?
2 ATP + 3 L-Leu	2 ADP + 2 phosphate + L-leucyl-L-leucyl-L-leucine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetra- and pentamers of L-Leu	-	?
2 ATP + 3 L-Leu	2 ADP + 2 phosphate + L-leucyl-L-leucyl-L-leucine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Leu	-	?
2 ATP + 3 L-Leu	2 ADP + 2 phosphate + L-leucyl-L-leucyl-L-leucine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Leu	-	?
2 ATP + 3 L-Leu	2 ADP + 2 phosphate + L-leucyl-L-leucyl-L-leucine		Chromobacterium violaceum	-	-	714524	products are di-, trimers of L-Leu	-	?
2 ATP + 3 L-Leu	2 ADP + 2 phosphate + L-leucyl-L-leucyl-L-leucine		Herpetosiphon aurantiacus	A9AVH5	-	714524	products are di-, trimers of L-Leu	-	?
2 ATP + 3 L-Met	2 ADP + 2 phosphate + L-methionyl-L-methionyl-L-methionine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Met	-	?
2 ATP + 3 L-Met	2 ADP + 2 phosphate + L-methionyl-L-methionyl-L-methionine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetra- and pentamers of L-Met	-	?
2 ATP + 3 L-Met	2 ADP + 2 phosphate + L-methionyl-L-methionyl-L-methionine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Met	-	?
2 ATP + 3 L-Met	2 ADP + 2 phosphate + L-methionyl-L-methionyl-L-methionine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Met	-	?
2 ATP + 3 L-Met	2 ADP + 2 phosphate + L-methionyl-L-methionyl-L-methionine		Chromobacterium violaceum	-	-	714524	products are di-, trimers of L-Met	-	?
2 ATP + 3 L-Met	2 ADP + 2 phosphate + L-methionyl-L-methionyl-L-methionine		Herpetosiphon aurantiacus	A9AVH5	-	714524	products are di-, trimers of L-Met	-	?
2 ATP + 3 L-Phe	2 ADP + 2 phosphate + L-phenylalanyl-L-phenylalanyl-L-phenylalanine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra- and pentamers of L-Phe	-	?
2 ATP + 3 L-Phe	2 ADP + 2 phosphate + L-phenylalanyl-L-phenylalanyl-L-phenylalanine		Herpetosiphon aurantiacus	A9AVH5	-	714524	products are di-, trimers of L-Phe	-	?
2 ATP + 3 L-Phe	2 ADP + 2 phosphate + L-phenylalanyl-L-phenylalanyl-L-phenylalanine		Bacillus licheniformis	Q65P25	-	714524	products are dimers and trimers of L-Phe	-	?
2 ATP + 3 L-Trp	2 ADP + 2 phosphate + L-tryptophanyl-L-tryptophanyl-L-tryptophan		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra- and pentamers of L-Trp	-	?
2 ATP + 3 L-Trp	2 ADP + 2 phosphate + L-tryptophanyl-L-tryptophanyl-L-tryptophan		Bacillus licheniformis	Q65P25	-	714524	products are dimers and trimers of L-Trp	-	?
2 ATP + 3 L-Tyr	2 ADP + 2 phosphate + L-tyrosyl-L-tyrosyl-L-tyrosine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra- and pentamers of L-Tyr	-	?
2 ATP + 3 L-Val	2 ADP + 2 phosphate + L-valyl-L-valyl-L-valine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Val	-	?
2 ATP + 3 L-Val	2 ADP + 2 phosphate + L-valyl-L-valyl-L-valine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetra- and pentamers of L-Val	-	?
2 ATP + 3 L-Val	2 ADP + 2 phosphate + L-valyl-L-valyl-L-valine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Val	-	?
2 ATP + 3 L-Val	2 ADP + 2 phosphate + L-valyl-L-valyl-L-valine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra-, pentamers of L-Val	-	?
2 ATP + 3 L-Val	2 ADP + 2 phosphate + L-valyl-L-valyl-L-valine		Chromobacterium violaceum	-	-	714524	products are di-, trimers of L-Val	-	?
2 ATP + 3 L-Val	2 ADP + 2 phosphate + L-valyl-L-valyl-L-valine		Herpetosiphon aurantiacus	A9AVH5	-	714524	products are di-, trimers of L-Val	-	?
3 ATP + 4 L-Ile	3 ADP + 2 phosphate + L-isoleucyl-L-isoleucyl-L-isoleucyl-L-isoleucine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetramers of L-Ile	-	?
3 ATP + 4 L-Ile	3 ADP + 3 phosphate + L-isoleucyl-L-isoleucyl-L-isoleucyl-L-isoleucine		Chromobacterium violaceum, Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Ile	-	?
3 ATP + 4 L-Ile	3 ADP + 3 phosphate + L-isoleucyl-L-isoleucyl-L-isoleucyl-L-isoleucine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra- and pentamers of L-Ile	-	?
3 ATP + 4 L-Ile	3 ADP + 3 phosphate + L-isoleucyl-L-isoleucyl-L-isoleucyl-L-isoleucine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetramers of L-Ile	-	?
3 ATP + 4 L-Leu	3 ADP + 3 phosphate + L-leucyl-L-leucyl-L-leucyl-L-leucine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Leu	-	?
3 ATP + 4 L-Leu	3 ADP + 3 phosphate + L-leucyl-L-leucyl-L-leucyl-L-leucine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetra- and pentamers of L-Leu	-	?
3 ATP + 4 L-Leu	3 ADP + 3 phosphate + L-leucyl-L-leucyl-L-leucyl-L-leucine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Leu	-	?
3 ATP + 4 L-Leu	3 ADP + 3 phosphate + L-leucyl-L-leucyl-L-leucyl-L-leucine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Leu	-	?
3 ATP + 4 L-Met	3 ADP + 3 phosphate + L-methionyl-L-methionyl-L-methionyl-L-methionine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Met	-	?
3 ATP + 4 L-Met	3 ADP + 3 phosphate + L-methionyl-L-methionyl-L-methionyl-L-methionine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetra- and pentamers of L-Met	-	?
3 ATP + 4 L-Met	3 ADP + 3 phosphate + L-methionyl-L-methionyl-L-methionyl-L-methionine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Met	-	?
3 ATP + 4 L-Met	3 ADP + 3 phosphate + L-methionyl-L-methionyl-L-methionyl-L-methionine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Met	-	?
3 ATP + 4 L-Phe	3 ADP + 3 phosphate + L-phenylalanyl-L-phenylalanyl-L-phenylalanyl-L-phenylalanine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra- and pentamers of L-Phe	-	?
3 ATP + 4 L-Trp	3 ADP + 3 phosphate + L-tryptophanyl-L-tryptophanyl-L-tryptophanyl-L-tryptophan		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra- and pentamers of L-Trp	-	?
3 ATP + 4 L-Tyr	3 ADP + 3 phosphate + L-tyrosyl-L-tyrosyl-L-tyrosyl-L-tyrosine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra- and pentamers of L-Tyr	-	?
3 ATP + 4 L-Val	3 ADP + 3 phosphate + L-valyl-L-valyl-L-valyl-L-valine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Val	-	?
3 ATP + 4 L-Val	3 ADP + 3 phosphate + L-valyl-L-valyl-L-valyl-L-valine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetra- and pentamers of L-Val	-	?
3 ATP + 4 L-Val	3 ADP + 3 phosphate + L-valyl-L-valyl-L-valyl-L-valine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Val	-	?
3 ATP + 4 L-Val	3 ADP + 3 phosphate + L-valyl-L-valyl-L-valyl-L-valine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra-, pentamers of L-Val	-	?
4 ATP + 5 L-Ile	4 ADP + 3 phosphate + L-isoleucyl-L-isoleucyl-L-isoleucyl-L-isoleucyl-L-isoleucine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Ile	-	?
4 ATP + 5 L-Leu	4 ADP + 4 phosphate + L-leucyl-L-leucyl-L-leucyl-L-leucine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Leu	-	?
4 ATP + 5 L-Leu	4 ADP + 4 phosphate + L-leucyl-L-leucyl-L-leucyl-L-leucine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Leu	-	?
4 ATP + 5 L-Leu	4 ADP + 4 phosphate + L-leucyl-L-leucyl-L-leucyl-L-leucyl-L-leucine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Leu	-	?
4 ATP + 5 L-Leu	4 ADP + 4 phosphate + L-leucyl-L-leucyl-L-leucyl-L-leucyl-L-leucine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetra- and pentamers of L-Leu	-	?
4 ATP + 5 L-Met	4 ADP + 4 phosphate + L-methionyl-L-methionyl-L-methionyl-L-methionyl-L-methionine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Met	-	?
4 ATP + 5 L-Met	4 ADP + 4 phosphate + L-methionyl-L-methionyl-L-methionyl-L-methionyl-L-methionine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetra- and pentamers of L-Met	-	?
4 ATP + 5 L-Met	4 ADP + 4 phosphate + L-methionyl-L-methionyl-L-methionyl-L-methionyl-L-methionine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Met	-	?
4 ATP + 5 L-Met	4 ADP + 4 phosphate + L-methionyl-L-methionyl-L-methionyl-L-methionyl-L-methionine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Met	-	?
4 ATP + 5 L-Phe	4 ADP + 4 phosphate + L-phenylalanyl-L-phenylalanyl-L-phenylalanyl-L-phenylalanyl-L-phenylalanine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra- and pentamers of L-Phe	-	?
4 ATP + 5 L-Trp	4 ADP + 4 phosphate + L-tryptophanyl-L-tryptophanyl-L-tryptophanyl-L-tryptophanyl-L-tryptophan		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra- and pentamers of L-Trp	-	?
4 ATP + 5 L-Tyr	4 ADP + 4 phosphate + L-tyrosyl-L-tyrosyl-L-tyrosyl-L-tyrosyl-L-tyrosine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra- and pentamers of L-Tyr	-	?
4 ATP + 5 L-Val	4 ADP + 4 phosphate + L-valyl-L-valyl-L-valyl-L-valyl-L-valine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Val	-	?
4 ATP + 5 L-Val	4 ADP + 4 phosphate + L-valyl-L-valyl-L-valyl-L-valyl-L-valine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetra- and pentamers of L-Val	-	?
4 ATP + 5 L-Val	4 ADP + 4 phosphate + L-valyl-L-valyl-L-valyl-L-valyl-L-valine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Val	-	?
4 ATP + 5 L-Val	4 ADP + 4 phosphate + L-valyl-L-valyl-L-valyl-L-valyl-L-valine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra-, pentamers of L-Val	-	?
5 ATP + 6 L-Leu	5 ADP + 2 phosphate + L-leucyl-L-leucyl-L-leucyl-L-leucyl-L-leucyl-L-leucine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Leu	-	?
5 ATP + 6 L-Leu	5 ADP + 5 phosphate + L-leucyl-L-leucyl-L-leucyl-L-leucyl-L-leucyl-L-leucine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Leu	-	?
5 ATP + 6 L-Met	5 ADP + 5 phosphate + L-methionyl-L-methionyl-L-methionyl-L-methionyl-L-methionyl-L-methionine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Met	-	?
5 ATP + 6 L-Met	5 ADP + 5 phosphate + L-methionyl-L-methionyl-L-methionyl-L-methionyl-L-methionyl-L-methionine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Met	-	?
5 ATP + 6 L-Val	5 ADP + 5 phosphate + L-valyl-L-valyl-L-valyl-L-valyl-L-valyl-L-valine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Val	-	?
ATP + 2 L-Ile	ADP + phosphate + L-isoleucyl-L-isoleucine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Ile	-	?
ATP + 2 L-Ile	ADP + phosphate + L-isoleucyl-L-isoleucine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetramers of L-Ile	-	?
ATP + 2 L-Ile	ADP + phosphate + L-isoleucyl-L-isoleucine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetramers of L-Ile	-	?
ATP + 2 L-Ile	ADP + phosphate + L-isoleucyl-L-isoleucine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetramers of L-Ile	-	?
ATP + 2 L-Ile	ADP + phosphate + L-isoleucyl-L-isoleucine		Herpetosiphon aurantiacus	A9AVH5	-	714524	products are di-, trimers of L-Ile	-	?
ATP + 2 L-Ile	ADP + phosphate + L-isoleucyl-L-isoleucine		Chromobacterium violaceum	-	-	714524	products are dimers of L-Ile	-	?
ATP + 2 L-Leu	ADP + phosphate + L-leucyl-L-leucine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Leu	-	?
ATP + 2 L-Leu	ADP + phosphate + L-leucyl-L-leucine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetra- and pentamers of L-Leu	-	?
ATP + 2 L-Leu	ADP + phosphate + L-leucyl-L-leucine		Herpetosiphon aurantiacus	A9AVH5	-	714524	products are di-, tri-, tetra- and pentamers of L-Leu	-	?
ATP + 2 L-Leu	ADP + phosphate + L-leucyl-L-leucine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Leu	-	?
ATP + 2 L-Leu	ADP + phosphate + L-leucyl-L-leucine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Leu	-	?
ATP + 2 L-Leu	ADP + phosphate + L-leucyl-L-leucine		Chromobacterium violaceum	-	-	714524	products are di-, trimers of L-Leu	-	?
ATP + 2 L-Met	ADP + phosphate + L-methionyl-L-methionine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Met	-	?
ATP + 2 L-Met	ADP + phosphate + L-methionyl-L-methionine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetra- and pentamers of L-Met	-	?
ATP + 2 L-Met	ADP + phosphate + L-methionyl-L-methionine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Met	-	?
ATP + 2 L-Met	ADP + phosphate + L-methionyl-L-methionine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Met	-	?
ATP + 2 L-Met	ADP + phosphate + L-methionyl-L-methionine		Chromobacterium violaceum	-	-	714524	products are di-, trimers of L-Met	-	?
ATP + 2 L-Met	ADP + phosphate + L-methionyl-L-methionine		Herpetosiphon aurantiacus	A9AVH5	-	714524	products are di-, trimers of L-Met	-	?
ATP + 2 L-Phe	ADP + phosphate + L-phenylalanyl-L-phenylalanine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra- and pentamers of L-Phe	-	?
ATP + 2 L-Phe	ADP + phosphate + L-phenylalanyl-L-phenylalanine		Herpetosiphon aurantiacus	A9AVH5	-	714524	products are di-, trimers of L-Phe	-	?
ATP + 2 L-Phe	ADP + phosphate + L-phenylalanyl-L-phenylalanine		Bacillus licheniformis	Q65P25	-	714524	products are dimers and trimers of L-Phe	-	?
ATP + 2 L-Phe	ADP + phosphate + L-phenylalanyl-L-phenylalanine		Streptococcus pneumoniae	-	-	714524	products are dimers of L-Phe	-	?
ATP + 2 L-Trp	ADP + phosphate + L-tryptophanyl-L-tryptophan		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra- and pentamers of L-Trp	-	?
ATP + 2 L-Trp	ADP + phosphate + L-tryptophanyl-L-tryptophan		Bacillus licheniformis	Q65P25	-	714524	products are dimers and trimers of L-Trp	-	?
ATP + 2 L-Trp	ADP + phosphate + L-tryptophanyl-L-tryptophan		Bacillus subtilis, Streptococcus pneumoniae	-	-	714524	products are dimers L-Trp	-	?
ATP + 2 L-Trp	ADP + phosphate + L-tryptophanyl-L-tryptophan		Herpetosiphon aurantiacus	A9AVH5	-	714524	products are dimers L-Trp	-	?
ATP + 2 L-Trp	ADP + phosphate + L-tryptophanyl-L-tryptophan		Chromobacterium violaceum	-	-	714524	products are dimers of L-Trp	-	?
ATP + 2 L-Tyr	ADP + phosphate + L-tyrosyl-L-tyrosine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra- and pentamers of L-Tyr	-	?
ATP + 2 L-Val	ADP + phosphate + L-valyl-L-valine		Bacillus subtilis	-	-	714524	products are di-, tri-, tetra- and pentamers of L-Val	-	?
ATP + 2 L-Val	ADP + phosphate + L-valyl-L-valine		Bacillus licheniformis	Q65P25	-	714524	products are di-, tri-, tetra- and pentamers of L-Val	-	?
ATP + 2 L-Val	ADP + phosphate + L-valyl-L-valine		Streptococcus pneumoniae	-	-	714524	products are di-, tri-, tetra-, penta- and hexamers of L-Val	-	?
ATP + 2 L-Val	ADP + phosphate + L-valyl-L-valine		Bifidobacterium adolescentis	A1A2P8	-	714524	products are di-, tri-, tetra-, pentamers of L-Val	-	?
ATP + 2 L-Val	ADP + phosphate + L-valyl-L-valine		Chromobacterium violaceum	-	-	714524	products are di-, trimers of L-Val	-	?
ATP + 2 L-Val	ADP + phosphate + L-valyl-L-valine		Herpetosiphon aurantiacus	A9AVH5	-	714524	products are di-, trimers of L-Val	-	?
ATP + alanine + alanine	ADP + phosphate + L-alanyl-L-alanine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + an L-amino acid + an L-amino acid	ADP + phosphate + L-aminoacyl-L-amino acid		Ralstonia solanacearum	B2DEW2	substrate and dipeptide formation specificity analysis, the enzyme shows high activity with L-Phe, L-Met, and L-His, detailed overview. RSp1486a exhibits different substrate specificity from Bacillus subtilus Lal, and preferably synthesizes hetero-dipeptides where a more bulky amino acid is placed at the N-terminus and a less bulky amino acid is placed at the C-terminus in contrast to those synthesized by Bacillus subtilus Lal, overview	690733	-	-	?
ATP + an L-amino acid + an L-amino acid	ADP + phosphate + L-aminoacyl-L-aminoacid		Bacillus subtilis	B5UAT8	assay at pH 8.0, 30�C	702767	-	-	?
ATP + an L-amino acid + an L-amino acid	ADP + phosphate + _aminoacyl-L-amino acid		Bacillus subtilis	C0STU2	assay at pH 8.0, 30�C	702768	-	-	?
ATP + arginine + histidine	ADP + phosphate + L-arginyl-L-histidine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + arginine + methionine	ADP + phosphate + L-arginyl-L-methionine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + arginine + phenylalanine	ADP + phosphate + L-arginyl-L-phenylalanine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + arginine + phenylalanine	ADP + phosphate + L-arginyl-L-phenylalanine		Streptococcus pneumoniae	Q97RD7	assay at pH 8.0, 37�C	702771	-	-	?
ATP + arginine + tryptophan	ADP + phosphate + L-arginyl-L-tryptophan		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + arginine + tryptophan	ADP + phosphate + L-arginyl-L-tryptophan		Streptococcus pneumoniae	Q97RD7	assay at pH 8.0, 37�C	702771	-	-	?
ATP + arginine + tyrosine	ADP + phosphate + L-arginyl-L-tyrosine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + asparagine + leucine	ADP + phosphate + L-asparagyl-L-leucine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + asparagine + phenylalanine	ADP + phosphate + L-asparagyl-L-phenylalanine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + asparagine + tryptophan	ADP + phosphate + L-asparagyl-L-tryptophan		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + cysteine + alanine	ADP + phosphate + L-cysteinyl-L-alanine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + cysteine + cysteine	ADP + phosphate + L-cysteinyl-L-cysteine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + cysteine + glutamine acid	ADP + phosphate +		Treponema denticola	Q73KK9	assay at pH 8.0, 37�C	702771	-	-	?
ATP + cysteine + glutamine acid	ADP + phosphate + ?		Photorhabdus luminescens subsp. laumondii	Q7N7D2	assay at pH 8.0, 37�C	702771	-	-	?
ATP + cysteine + serine	ADP + phosphate + L-cysteinyl-L-serine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + cysteine + threonine	ADP + phosphate + L-cysteinyl-L-threonine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + glutamine + alanine	ADP + phosphate + L-glutamyl-L-alanine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + glutamine + glutamine acid	ADP + phosphate + ?		Photorhabdus luminescens subsp. laumondii	Q7N7D2	assay at pH 8.0, 37�C	702771	-	-	?
ATP + glutamine + leucine	ADP + phosphate + L-glutamyl-L-leucine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + glutamine + methionine	ADP + phosphate + L-glutamyl-L-methionine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + glutamine + phenylalanine	ADP + phosphate + L-glutaminyl-L-phenylalanine		Photorhabdus luminescens subsp. laumondii	Q7N7D2	assay at pH 8.0, 37�C	702771	-	-	?
ATP + glutamine + phenylalanine	ADP + phosphate + L-glutamyl-L-phenylalanine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + glutamine + phenylalanine	ADP + phosphate + L-gutamyl-L-phenylalanine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + glutamine + tryptophan	ADP + phosphate + L-glutamyl-tryptophan		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + glutamine acid + leucine	ADP + phosphate + ?		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + glycine + L-proline	ADP + phosphate + glycyl-L-proline		Treponema denticola	Q73KK9	assay at pH 8.0, 37�C	702771	-	-	?
ATP + histidine + alanine	ADP + phosphate + L-histidyl-L-alanine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + histidine + asparagine	ADP + phosphate + L-histidyl-L-asparagine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + histidine + cysteine	ADP + phosphate + L-histidyl-L-cysteine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + histidine + glutamine	ADP + phosphate + L-histidyl-L-glutamine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + histidine + histidine	ADP + phosphate + L-histidyl-L-histidine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + histidine + methionine	ADP + phosphate + L-histidyl-L-methionine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + histidine + phenylalanine	ADP + phosphate + L-histidyl-L-phenylalanine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + histidine + phenylalanine	ADP + phosphate + L-histidyl-L-phenylalanine		Streptococcus pneumoniae	Q97RD7	assay at pH 8.0, 37�C	702771	-	-	?
ATP + histidine + proline	ADP + phosphate + L-histidyl-L-proline		Treponema denticola	Q73KK9	assay at pH 8.0, 37�C	702771	-	-	?
ATP + histidine + proline	ADP + phosphate + L-histidyl-L-proline		Streptococcus pneumoniae	Q97RD7	assay at pH 8.0, 37�C	702771	-	-	?
ATP + histidine + serine	ADP + phosphate + L-histidyl-L-serine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + histidine + tryptophan	ADP + phosphate + L-histidyl-L-tryptophan		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + histidine + valine	ADP + phosphate + L-histidyl-L-valine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + isoleucine + alanine	ADP + phosphate + L-isoleucyl-L-alanine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + isoleucine + cysteine	ADP + phosphate + L-isoleucyl-L-cysteine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + isoleucine + serine	ADP + phosphate + L-isoleucyl-L-serine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + L-arginine + L-alanine	ADP + phosphate + L-arginyl-L-alanine		Bacillus subtilis	B5UAT8	assay at pH 8.0, 30�C	702767	-	-	?
ATP + L-arginine + L-arginine	ADP + phosphate + L-arginyl-L-arginine		Bacillus subtilis	B5UAT8	assay at pH 8.0, 30�C	702767	-	-	?
ATP + L-arginine + L-histidine	ADP + phosphate + L-arginyl-L-histidine		Bacillus subtilis	B5UAT8	assay at pH 8.0, 30�C	702767	-	-	?
ATP + L-arginine + L-serine	ADP + phosphate + L-arginyl-L-serine		Bacillus subtilis	B5UAT8	assay at pH 8.0, 30�C	702767	-	-	?
ATP + L-asparagine + L-methionine	ADP + phosphate + L-asparagyl-L-methionine		Bacillus licheniformis	-	-	693286	-	-	?
ATP + L-cysteine + glycine	ADP + phosphate + L-cysteinyl-glycine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + L-histidine + glycine	ADP + phosphate + L-histidyl-glycine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + L-leucine + an L-amino acid	ADP + phosphate + L-leucyl-L-amino acid		Bacillus licheniformis	-	the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview	693286	-	-	?
ATP + L-leucine + glycine	ADP + phosphate + L-leucyl-glycine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + L-leucine + glycine	ADP + phosphate + L-leucyl-glycine		Streptococcus pneumoniae	Q97RD7	assay at pH 8.0, 37�C	702771	-	-	?
ATP + L-leucine + L-alanine	ADP + phosphate + L-leucyl-L-alanine		Bacillus licheniformis	-	-	693286	-	-	?
ATP + L-methionine + an L-amino acid	ADP + phosphate + L-methionyl-L-amino acid		Bacillus licheniformis	-	the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview	693286	-	-	?
ATP + L-methionine + glycine	ADP + phosphate + L-methionyl-glycine		Bacillus licheniformis	-	-	693286	-	-	?
ATP + L-methionine + glycine	ADP + phosphate + L-methionyl-glycine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + L-methionine + L-alanine	ADP + phosphate + L-methionyl-L-alanine		Bacillus licheniformis	-	best substrate combination	693286	-	-	?
ATP + L-methionine + L-cysteine	ADP + phosphate + L-methionyl-L-cysteine		Bacillus licheniformis	-	-	693286	-	-	?
ATP + L-methionine + L-leucin	ADP + phosphate + L-methionyl-L-leucine		Bacillus licheniformis	-	-	693286	-	-	?
ATP + L-methionine + L-methionine	ADP + phosphate + L-methionyl-L-methionine		Bacillus licheniformis	-	-	693286	-	-	?
ATP + L-phenylalanine + glycine	ADP + phosphate + L-phenylalanyl-glycine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + L-phenylalanine + glycine	ADP + phosphate + L-phenylalanyl-glycine		Streptococcus pneumoniae	Q97RD7	assay at pH 8.0, 37�C	702771	-	-	?
ATP + L-serine + L-methionine	ADP + phosphate + L-seryl-L-methionine		Bacillus licheniformis	-	-	693286	-	-	?
ATP + L-threonine + L-methionine	ADP + phosphate + L-threonyl-L-methionine		Bacillus licheniformis	-	-	693286	-	-	?
ATP + L-tryptophan + glycine	ADP + phosphate + L-tryptophanyl-glycine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + L-tryptophan + glycine	ADP + phosphate + L-tryptophanyl-glycine		Streptococcus pneumoniae	Q97RD7	assay at pH 8.0, 37�C	702771	-	-	?
ATP + L-tyrosine + glycine	ADP + phosphate + L-tyrosinyl-glycine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + leucine + alanine	ADP + phosphate + L-leucyl-L-alanine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + leucine + cysteine	ADP + phosphate + L-leucyl-L-cysteine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + leucine + serine	ADP + phosphate + L-leucyl-L-serine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + leucine + threonine	ADP + phosphate + L-leucyl-L-threonine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + lysine + histidine	ADP + phosphate + L-lysyl-L-histidine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + lysine + phenylalanine	ADP + phosphate + L-lysyl-L-phenylalanine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + methionine + alanine	ADP + phosphate + L-methionyl-L-alanine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + methionine + cysteine	ADP + phosphate + L-methionyl-L-cysteine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + methionine + isoleucine	ADP + phosphate + L-methionyl-L-isoleucine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + methionine + leucine	ADP + phosphate + L-methionyl-L-leucine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + methionine + methionine	ADP + phosphate + L-methionyl-L-methionine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + methionine + serine	ADP + phosphate + L-methionyl-L-serine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + methionine + threonine	ADP + phosphate + L-methionyl-L-threonine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + methionine + valine	ADP + phosphate + L-methionyl-L-valine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + phenylalanine + alanine	ADP + phosphate + L-phenylalanyl-L-alanine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + phenylalanine + cysteine	ADP + phosphate + L-phenylalanyl-L-cysteine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + phenylalanine + cysteine	ADP + phosphate + L-phenylalanyl-L-cysteine		Streptococcus pneumoniae	Q97RD7	assay at pH 8.0, 37�C	702771	-	-	?
ATP + phenylalanine + isoleucine	ADP + phosphate + L-phenylalanyl-L-isoleucine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + phenylalanine + methionine	ADP + phosphate + L-phenylalanyl-L-methionine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + phenylalanine + phenylalanine	ADP + phosphate + L-phenylalanyl-L-phenylalanine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + phenylalanine + phenylalanine	ADP + phosphate + L-phenylalanyl-L-phenylalanine		Streptococcus pneumoniae	Q97RD7	assay at pH 8.0, 37�C	702771	-	-	?
ATP + phenylalanine + serine	ADP + phosphate + L-phenylalanyl-L-serine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + phenylalanine + threonine	ADP + phosphate + L-phenylalanyl-L-threonine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + phenylalanine + valine	ADP + phosphate + L-phenylalanyl-L-valine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + serine + alanine	ADP + phosphate + L-serinyl-L-alanine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + serine + serine	ADP + phosphate + L-serinyl-L-serine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tryptophan + alanine	ADP + phosphate + L-tryptophanyl-L-alanine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tryptophan + alanine	ADP + phosphate + L-tryptophanyl-L-alanine		Streptococcus pneumoniae	Q97RD7	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tryptophan + cysteine	ADP + phosphate + L-tryptophanyl-L-cysteine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tryptophan + cysteine	ADP + phosphate + L-tryptophanyl-L-cysteine		Streptococcus pneumoniae	Q97RD7	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tryptophan + isoleucine	ADP + phosphate + L-tryptophanyl-L-isoleucine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tryptophan + leucine	ADP + phosphate + L-tryptophanyl-L-leucine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tryptophan + methionine	ADP + phosphate + L-tryptophanyl-L-methionine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tryptophan + phenylalanine	ADP + phosphate + L-tryptophanyl-L-phenylalanine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tryptophan + serine	ADP + phosphate + L-tryptophanyl-L-serine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tryptophan + threonine	ADP + phosphate + L-tryptophanyl-L-threonine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tryptophan + tryptophan	ADP + phosphate + L-tryptophanyl-L-tryptophan		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tryptophan + valine	ADP + phosphate + L-tryptophanyl-L-valine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tyrosine + alanine	ADP + phosphate + L-tyrosinyl-L-alanine		Streptococcus mutans, Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tyrosine + cysteine	ADP + phosphate + L-tyrosinyl-L-cysteine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tyrosine + methionine	ADP + phosphate + L-tyrosinyl-L-methionine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tyrosine + phenylalanine	ADP + phosphate + L-tyrosinyl-L-phenylalanine		Actinobacillus pleuropneumoniae	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + tyrosine + serine	ADP + phosphate + L-tyrosinyl-L-serine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + valine + alanine	ADP + phosphate + L-valinyl-L-alanine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
ATP + valine + serine	ADP + phosphate + L-valinyl-L-serine		Streptococcus mutans	-	assay at pH 8.0, 37�C	702771	-	-	?
additional information	?	-	Bacillus licheniformis	-	the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is very strict, overview	693286	-	-	-

NATURAL SUBSTRATES	NATURAL PRODUCTS	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY SUBSTRATE	LITERATURE (Substrate)	COMMENTARY PRODUCT	LITERATURE (Product)
ATP + an L-amino acid + an L-amino acid	ADP + phosphate + L-aminoacyl-L-amino acid		Ralstonia solanacearum	B2DEW2	-	690733	-	-
ATP + L-leucine + an L-amino acid	ADP + phosphate + L-leucyl-L-amino acid		Bacillus licheniformis	-	the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview	693286	-	-
ATP + L-methionine + an L-amino acid	ADP + phosphate + L-methionyl-L-amino acid		Bacillus licheniformis	-	the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview	693286	-	-

COFACTOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
ATP	Ralstonia solanacearum	B2DEW2	-	690733	2D-image
ATP	Bacillus licheniformis	-	-	693286	2D-image

METALS and IONS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
Mg2+	Ralstonia solanacearum	B2DEW2	-	690733
Mg2+	Bacillus licheniformis	-	-	693286
Mg2+	Bacillus subtilis	B5UAT8	essential for enzyme activity	702767
Mg2+	Bacillus subtilis	C0STU2	required for enzyme activity	702768

INHIBITORS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

ACTIVATING COMPOUND	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

KM VALUE [mM]	KM VALUE [mM] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
additional information	-	additional information	Ralstonia solanacearum	B2DEW2	Michaelis-Menten kinetics	690733	-

TURNOVER NUMBER [1/s]	TURNOVER NUMBER MAXIMUM[1/s]	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

kcat/KM VALUE [1/mMs^-1]	kcat/KM VALUE [1/mMs^-1] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

Ki VALUE [mM]	Ki VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

IC50 VALUE [mM]	IC50 VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg]	SPECIFIC ACTIVITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
0.00158	-	Bacillus subtilis	B5UAT8	crude enzyme in cell-free extract	702767
2.02	-	Bacillus subtilis	B5UAT8	enzyme after total purification with precipitation and columns	702767

pH OPTIMUM	pH MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
8	-	Ralstonia solanacearum	B2DEW2	assay at	690733
8	-	Bacillus subtilis	C0STU2	assay at	702768
8	-	Actinobacillus pleuropneumoniae	-	assay at	702771
8	-	Photorhabdus luminescens subsp. laumondii	Q7N7D2	assay at	702771
8	-	Streptococcus mutans	-	assay at	702771
8	-	Streptococcus pneumoniae	Q97RD7	assay at	702771
8	-	Treponema denticola	Q73KK9	assay at	702771
9	-	Bacillus licheniformis	-	-	693286
9.5	-	Bacillus subtilis	B5UAT8	-	702767

pH RANGE	pH RANGE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
6	10	Ralstonia solanacearum	B2DEW2	-	690733
7	11	Bacillus subtilis	B5UAT8	enzyme activity decreases sharply below 9.0 and above 10.0	702767
9	9.5	Bacillus subtilis	C0STU2	enzyme activity decreased sharply above pH 10.0	702768

TEMPERATURE OPTIMUM	TEMPERATURE OPTIMUM MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
37	-	Ralstonia solanacearum	B2DEW2	assay at	690733
37	-	Bacillus licheniformis	-	-	693286
37	-	Bacillus subtilis	B5UAT8	-	702767
37	-	Actinobacillus pleuropneumoniae	-	assay at	702771
37	-	Photorhabdus luminescens subsp. laumondii	Q7N7D2	assay at	702771
37	-	Streptococcus mutans	-	assay at	702771
37	-	Streptococcus pneumoniae	Q97RD7	assay at	702771
37	-	Treponema denticola	Q73KK9	assay at	702771
40	-	Bacillus subtilis	C0STU2	-	702768

TEMPERATURE RANGE	TEMPERATURE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
20	50	Bacillus subtilis	C0STU2	significant decrease in activity above 45�C	702768
20	60	Bacillus subtilis	B5UAT8	significant decrease above 50�C	702767

pI VALUE	pI VALUE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

SOURCE TISSUE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	SOURCE
No entries in this field

LOCALIZATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	GeneOntology No.	LITERATURE	SOURCE
soluble	Bacillus subtilis	B5UAT8	-	-	702767

PDB	SCOP	CATH	ORGANISM
3vot, download	SCOP (3vot)	CATH (3vot)	Bacillus licheniformis (strain DSM 13 / ATCC 14580)
3vmm, download	SCOP (3vmm)	CATH (3vmm)	Bacillus subtilis (strain 168)

MOLECULAR WEIGHT	MOLECULAR WEIGHT MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
45000	-	Bacillus subtilis	B5UAT8	SDS-PAGE	702767
53500	-	Chromobacterium violaceum	-	gel filtration	714524
106500	-	Bacillus licheniformis	Q65P25	gel filtration	714524
109000	-	Bacillus subtilis	-	gel filtration	714524
115500	-	Herpetosiphon aurantiacus	A9AVH5	gel filtration	714524
126000	-	Streptococcus pneumoniae	-	gel filtration	714524
129000	-	Bifidobacterium adolescentis	A1A2P8	gel filtration	714524

SUBUNITS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
?	Ralstonia solanacearum	B2DEW2	x * 49500, recombinant His-tagged enzyme, SDS-PAGE	690733
dimer	Bacillus licheniformis	Q65P25	2 * 47000, calculated	714524
dimer	Bacillus subtilis	-	2 * 47000, calculated	714524
dimer	Bifidobacterium adolescentis	A1A2P8	2 * 49000, calculated	714524
dimer	Herpetosiphon aurantiacus	A9AVH5	2 * 51000, calculated	714524
dimer	Streptococcus pneumoniae	-	2 * 51000, calculated	714524
monomer	Chromobacterium violaceum	-	2 * 48000, calculated	714524

POSTTRANSLATIONAL MODIFICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

Crystallization/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

pH STABILITY	pH STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

TEMPERATURE STABILITY	TEMPERATURE STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

GENERAL STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

ORGANIC SOLVENT	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

OXIDATION STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

STORAGE STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

Purification/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
His Trap	Actinobacillus pleuropneumoniae	-	702771
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration	Bacillus licheniformis	-	693286
1200fold purification after (NH4)2SO4 precipitation, Butyl FF column, Mono Q column, Superdex column	Bacillus subtilis	B5UAT8	702767
His Trap	Photorhabdus luminescens subsp. laumondii	Q7N7D2	702771
recombinant His-tagged enzyme from Escherichia coli	Ralstonia solanacearum	B2DEW2	690733
His Trap	Streptococcus mutans	-	702771
His Trap	Streptococcus pneumoniae	Q97RD7	702771
His Trap	Treponema denticola	Q73KK9	702771

Cloned/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
expression in Escherichia coli	Actinobacillus pleuropneumoniae	-	702771
expression in Escherichia coli	Bacillus licheniformis	Q65P25	714524
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Bacillus licheniformis	-	693286
expression in Escherichia coli	Bacillus subtilis	-	714524
expression in Escherichia coli BL21	Bacillus subtilis	C0STU2	702768
expression in Escherichia coli rosetta	Bacillus subtilis	B5UAT8	702767
expression in Escherichia coli	Bifidobacterium adolescentis	A1A2P8	714524
expression in Escherichia coli	Chromobacterium violaceum	-	714524
expression in Escherichia coli	Herpetosiphon aurantiacus	A9AVH5	714524
expression in Escherichia coli	Photorhabdus luminescens subsp. laumondii	Q7N7D2	702771
gene lal, expression of His-tagged enzyme in Escherichia coli	Ralstonia solanacearum	B2DEW2	690733
expression in Escherichia coli	Streptococcus mutans	-	702771
expression in Escherichia coli	Streptococcus pneumoniae	-	702771, 714524
expression in Escherichia coli	Treponema denticola	Q73KK9	702771

EXPRESSION	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

ENGINEERING	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

Renatured/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

APPLICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
synthesis	Bacillus subtilis	-	fermentative production of L-alanyl-L-glutamine by a metabolically engineered Escherichia coli strain expressing L-amino acid alpha-ligase	671498

REF.	AUTHORS	TITLE	JOURNAL	VOL.	PAGES	YEAR	ORGANISM	LINK TO PUBMED	SOURCE
662037	Tabata, K.; Ikeda, H.; Hashimoto, S.	ywf in Bacillus subtilis codes for a novel enzyme, L-amino acid ligase	J. Bacteriol.	187	5195-5202	2005	Bacillus subtilis	PubMed
671498	Tabata, K.; Hashimoto, S.	Fermentative production of L-alanyl-L-glutamine by a metabolically engineered Escherichia coli strain expressing L-amino acid alpha-ligase	Appl. Environ. Microbiol.	73	6378-6385	2007	Bacillus subtilis	PubMed
690733	Kino, K.; Nakazawa, Y.; Yagasaki, M.	Dipeptide synthesis by L-amino acid ligase from Ralstonia solanacearum	Biochem. Biophys. Res. Commun.	371	536-540	2008	Ralstonia solanacearum	PubMed
693286	Kino, K.; Noguchi, A.; Nakazawa, Y.; Yagasaki, M.	A novel L-amino acid ligase from Bacillus licheniformis	J. Biosci. Bioeng.	106	313-315	2008	Bacillus licheniformis	PubMed
702767	Kino, K.; Kotanaka, Y.; Arai, T.; Yagasaki, M.	A novel L-amino acid ligase from Bacillus subtilis NBRC3134, a microorganism producing peptide-antibiotic rhizocticin	Biosci. Biotechnol. Biochem.	73	901-907	2009	Bacillus subtilis	PubMed
702768	Kino, K.; Arai, T.; Tateiwa, D.	A novel L-amino acid ligase from Bacillus subtilis NBRC3134 catalyzed oligopeptide synthesis	Biosci. Biotechnol. Biochem.	74	129-134	2010	Bacillus subtilis	PubMed
702771	Senoo, A.; Tabata, K.; Yonetani, Y.; Yagasaki, M.	Identification of novel L-amino acid alpha-ligases through hidden markov model-based profile analysis	Biosci. Biotechnol. Biochem.	74	415-418	2010	Actinobacillus pleuropneumoniae, Photorhabdus luminescens subsp. laumondii, Streptococcus mutans, Streptococcus pneumoniae, Treponema denticola	PubMed
714524	Arai, T.; Kino, K.	New L-amino acid ligases catalyzing oligopeptide synthesis from various microorganisms	Biosci. Biotechnol. Biochem.	74	1572-1577	2010	Bacillus licheniformis, Bacillus subtilis, Bifidobacterium adolescentis, Chromobacterium violaceum, Herpetosiphon aurantiacus, Streptococcus pneumoniae	PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 6.3.2.28)

NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

SYSTERS

Protein Mutant Database

InterPro (database of protein families, domains and functional sites)

All organisms
Actinobacillus pleuropneumoniae
Bacillus licheniformis
Bacillus licheniformis NBRC 12200
Bacillus licheniformis NBRC12200
Bacillus subtilis
Bacillus subtilis NBRC 3134
Bacillus subtilis NBRC3134
Bifidobacterium adolescentis
Bifidobacterium adolescentis JCM 1275
Chromobacterium violaceum
Chromobacterium violaceum NBRC 12614
Herpetosiphon aurantiacus
Herpetosiphon aurantiacus ATCC 23779
Photorhabdus luminescens subsp. laumondii
Ralstonia solanacearum
Ralstonia solanacearum JCM 10489
Streptococcus mutans
Streptococcus pneumoniae
Streptococcus pneumoniae ATCC BAA-255
Treponema denticola